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Team:Osaka/week8
From 2012.igem.org
(Difference between revisions)
| Line 3: | Line 3: | ||
==September 16 (Sun)== | ==September 16 (Sun)== | ||
| - | + | # Miniprep of yesterday's culture | |
| - | + | # Restriction digests of minipreppped parts | |
| + | # Gel electrophoresis of digests | ||
| + | # Gel extraction :161,162 | ||
| + | # Ligation | ||
| + | #* 163(C):161(upstream)+151(downstream)+1-3A(vector) | ||
| + | #* 164(C):161(upstream)+152(downstream)+1-3A(vector) | ||
| + | #* 165(C):162(upstream)+151(downstream)+1-3A(vector) | ||
| + | #* 166(C):162(upstream)+152(downstream)+1-3A(vector) | ||
| + | # Transformation of ligation products | ||
| + | # To liquid culture | ||
==September 17 (Mon)== | ==September 17 (Mon)== | ||
Revision as of 04:38, 15 September 2012
September 16 (Sun)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Gel extraction :161,162
- Ligation
- 163(C):161(upstream)+151(downstream)+1-3A(vector)
- 164(C):161(upstream)+152(downstream)+1-3A(vector)
- 165(C):162(upstream)+151(downstream)+1-3A(vector)
- 166(C):162(upstream)+152(downstream)+1-3A(vector)
- Transformation of ligation products
- To liquid culture
