"
Template:Team:TU Munich/Overview
From 2012.igem.org
(Difference between revisions)
| Line 145: | Line 145: | ||
<div class="sproj" id="light"> | <div class="sproj" id="light"> | ||
<h2><center>Light Switchable Promoter</center></h2> | <h2><center>Light Switchable Promoter</center></h2> | ||
| + | <br> | ||
<p>The idea behind a lightswitchable system is to create a gene expression system which can be induced and deactivated by light of a certain wavelengths.</p> | <p>The idea behind a lightswitchable system is to create a gene expression system which can be induced and deactivated by light of a certain wavelengths.</p> | ||
Revision as of 22:59, 14 September 2012
Regulation
Vector Design
To be able to test and quantify the expression of desired enzymes in yeast we designed an expression vector which is compatible to the iGEM RFC25 standard based on the commercially available pYES2 vector from Invitrogen.
Ethanol Inducible Promoter
The KlADH4-Promoter from the yeast ''Kluyveromyces lactis'' regulates the expression of a mitochondrial alcohol dehydrogenase in an ethanol-dependent way.
Light Switchable Promoter
The idea behind a lightswitchable system is to create a gene expression system which can be induced and deactivated by light of a certain wavelengths.
This system is extremely attractive, as induction does not require the addition of a specific substance. This makes induction cheap, fast, precise and also compatible to the bavarian purity law.
