Team:USTC-China

From 2012.igem.org

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<h3>WELCOME!</h3>
<h3>WELCOME!</h3>
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<p class="project">This year, USTC-China iGEMers are going to provide a novel immune system of engineering bacteria to fight against the bacteriophage. We hope this artificial line of defeat composed of gene circuits can be of practical use in fermentation industry.</p>  
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<p class="project">Bacteriophage is one of the most severe threats the fermentation factories have to face. To help solve the problem, we design a gene circuit which can both detect and defend against the phages. We use the modified promoter pRM to sense the phage’s infection and initiate the defence. The lysis gene which can make bacteria lyse is installed in our circuit. When it works, the phage won’t be able to take advantage of its host to replicate any longer. To win more time for lysis to function well, we design antisense RNA to prevent the phage from turning into lytic life cycle. Thus, when the lysis protein kills the host, the phage is still at lysogenic life cycle or the newly assembled phages are still immature. By using the quorum sensing system, the bacteria around the host will prepare to defend in advance. Attribute to these parts, our bacteria survive.</p>
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<img src="https://static.igem.org/mediawiki/2012/8/84/Project_overview.jpg" style="float:left;width:400px;height:300px;"  />
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<p class="project">Since the fermentation industry appeared, the phage infection has become a big threat to engineering bacteria. During mass production of dairy products, enzymes, amino acids, antibiotics and pesticides, the results would be unimaginable if bacteria were infected by bacteriophages. Bacteria will be abnormal and die and the infection will spread quickly. Without any efficient strategy, tremendous loss will lie ahead. Nowadays, common strategy for avoiding this catastrophe is to control the conditions of the fermentation strictly. Meanwhile, people keep selecting strains against phages. But all the strategies are time-consuming and far from once-for-all. </p>
 
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<img src="https://static.igem.org/mediawiki/2012/5/5e/Project_Map.jpg" style="float:left;width:55%;height:55%"  />
 
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<p class="project">However, the engineering bacteria which 2012 USTC-China iGEMers hope to cultivate would both protect themselves when bacteriophage invades the strain and focus on producing products without consuming extra energy when their living condition is safe. In this case, the quality of fermented products can be guaranteed and the profits of fermentation industry can be enormously improved. </p>
 
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<p class="button"><a href="https://2012.igem.org/Team:USTC-China/background">more</a></p>
<p class="button"><a href="https://2012.igem.org/Team:USTC-China/background">more</a></p>

Revision as of 15:13, 14 September 2012

WELCOME TO THE WIKI OF USTC-CHINA IGEM TAEM! ENJOY YOUR EXPLORATION!

PARTS

this summer we have attributed several parts to the partsregistry!

MODELING

We have theoretically analyse our system through Mathematical Modeling!

OUTREACH

Our team conduct several activities for further recognition of synthetic biology!

SAFETY

The project is considered to have the minimal risks!

TEAM

Members in USTC_China enjoy themselves this summer!

WELCOME!

Bacteriophage is one of the most severe threats the fermentation factories have to face. To help solve the problem, we design a gene circuit which can both detect and defend against the phages. We use the modified promoter pRM to sense the phage’s infection and initiate the defence. The lysis gene which can make bacteria lyse is installed in our circuit. When it works, the phage won’t be able to take advantage of its host to replicate any longer. To win more time for lysis to function well, we design antisense RNA to prevent the phage from turning into lytic life cycle. Thus, when the lysis protein kills the host, the phage is still at lysogenic life cycle or the newly assembled phages are still immature. By using the quorum sensing system, the bacteria around the host will prepare to defend in advance. Attribute to these parts, our bacteria survive.

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