Team:Osaka/week1
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==August 1 (Wed)== | ==August 1 (Wed)== | ||
# Preparation of LB agar plates (Amp) | # Preparation of LB agar plates (Amp) | ||
# Restriction digests of parts (See Table 1) | # Restriction digests of parts (See Table 1) | ||
+ | # Ligation | ||
+ | #* 143(A): 124+1-1G(vector) | ||
+ | #* 144(A): 125+1-1G(vector) | ||
+ | #* 145(A): 126+1-1G(vector) | ||
+ | #* 146(A): 127+1-1G(vector) | ||
+ | #* 147(A): 128+1-1G(vector) | ||
+ | #* 148(A): 129+1-1G(vector) | ||
+ | #* 149(A): 138+1-1G(vector) | ||
+ | #* 150(A): 139+1-1G(vector) | ||
+ | #* 151(A): 140+1-1G(vector) | ||
+ | #* 152(A): 141+1-1G(vector) | ||
+ | # Transformation of ligation products into DH5α <i>E.coli</i> | ||
+ | |||
{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5" | {|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5" | ||
|+Table 1 | |+Table 1 | ||
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|129||<bbpart>BBa_K602020</bbpart>||C|| <i>D. radiodurans</i> PprM+RecA expression device | |129||<bbpart>BBa_K602020</bbpart>||C|| <i>D. radiodurans</i> PprM+RecA expression device | ||
- | + | |- | |
+ | |138||<bbpart>BBa_K602005</bbpart>||C|| <i>D. radiodurans</i> PprI expression device | ||
+ | |- | ||
+ | |139||<bbpart>BBa_K602006</bbpart>||C|| <i>D. radiodurans</i> PprA expression device | ||
+ | |- | ||
+ | |140||<bbpart>BBa_K602007</bbpart>||C|| <i>D. radiodurans</i> PprM expression device | ||
+ | |- | ||
+ | |141||<bbpart>BBa_K602008</bbpart>||C|| <i>D. radiodurans</i> RecA expression device | ||
|} | |} | ||
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==August 2 (Thu) == | ==August 2 (Thu) == | ||
- | + | # Transfer to liquid culture:143~152 | |
+ | #* Some parts didn't successfully transformed | ||
==August 3 (Fri) == | ==August 3 (Fri) == | ||
+ | # Miniprep of yesterday's culture | ||
+ | # Restriction digests of minipreppped parts | ||
+ | # Gel electrophoresis of digests | ||
+ | # Transformation of ligation products into DH5α <i>E.coli</i> again:147,148,150 | ||
- | + | ==August 4 (Sat) == | |
- | ==August 4 ( | + | # Transfer to liquid culture |
- | + | # Restriction digests of 1-1G<vector> with SpeI,XbaI | |
+ | # Ligation | ||
+ | # Transformation of ligation products into DH5α <i>E.coli</i> | ||
+ | # Transformation of plasmid DNA into Rosetta <i>E.coli</i>:143~146,149,152 | ||
[[Team:Osaka/Notebook|Back to Notebook]] | [[Team:Osaka/Notebook|Back to Notebook]] |
Latest revision as of 01:33, 14 September 2012
August 1 (Wed)
- Preparation of LB agar plates (Amp)
- Restriction digests of parts (See Table 1)
- Ligation
- 143(A): 124+1-1G(vector)
- 144(A): 125+1-1G(vector)
- 145(A): 126+1-1G(vector)
- 146(A): 127+1-1G(vector)
- 147(A): 128+1-1G(vector)
- 148(A): 129+1-1G(vector)
- 149(A): 138+1-1G(vector)
- 150(A): 139+1-1G(vector)
- 151(A): 140+1-1G(vector)
- 152(A): 141+1-1G(vector)
- Transformation of ligation products into DH5α E.coli
number | Part Name | Resistance | Description |
---|---|---|---|
124 | <bbpart>BBa_K602015</bbpart> | C | D. radiodurans PprI+PprA expression device |
125 | <bbpart>BBa_K602016</bbpart> | C | D. radiodurans PprI+RecA expression device |
126 | <bbpart>BBa_K602017</bbpart> | C | D. radiodurans PprA+RecA expression device |
127 | <bbpart>BBa_K602018</bbpart> | C | D. radiodurans PprI+PprM expression device |
128 | <bbpart>BBa_K602019</bbpart> | C | D. radiodurans PprA+PprM expression device |
129 | <bbpart>BBa_K602020</bbpart> | C | D. radiodurans PprM+RecA expression device |
138 | <bbpart>BBa_K602005</bbpart> | C | D. radiodurans PprI expression device |
139 | <bbpart>BBa_K602006</bbpart> | C | D. radiodurans PprA expression device |
140 | <bbpart>BBa_K602007</bbpart> | C | D. radiodurans PprM expression device |
141 | <bbpart>BBa_K602008</bbpart> | C | D. radiodurans RecA expression device |
August 2 (Thu)
- Transfer to liquid culture:143~152
- Some parts didn't successfully transformed
August 3 (Fri)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Transformation of ligation products into DH5α E.coli again:147,148,150
August 4 (Sat)
- Transfer to liquid culture
- Restriction digests of 1-1G<vector> with SpeI,XbaI
- Ligation
- Transformation of ligation products into DH5α E.coli
- Transformation of plasmid DNA into Rosetta E.coli:143~146,149,152