Team:Osaka/week3

From 2012.igem.org

(Difference between revisions)
(Created page with "{{Osaka}} __NOTOC__ ==August 12 (Sun)== ==August 16 (Thu)== ==August 17 (Fri)== ==August 18 (Sat)== Back to Notebook")
 
(19 intermediate revisions not shown)
Line 1: Line 1:
-
{{Osaka}}
+
{{Osaka2}}
__NOTOC__
__NOTOC__
 +
==August 12 (Sun)==
==August 12 (Sun)==
-
 
+
# Miniprep of yesterday's culture
 +
# Restriction digests of minipreppped parts
 +
# Gel electrophoresis of digests
 +
# Ligation
 +
#* 153(C):1-2M(upstream)+1-10H(downstream)+1-1A(vector)
 +
#* 154(C):1-22A(upstream)+1-2M(downstream)+1-1A(vector)
 +
#* 155(C):1-2D(upstream)+1-23L(downstream)+1-1A(vector)
 +
# SDS-PAGE
 +
#* Electrophoresis
==August 16 (Thu)==
==August 16 (Thu)==
-
 
+
# Transformaton of ligation products into DH5α <i>E.coli</i>:153~155
 +
# Transformation of plasmid DNA into Rosetta <i>E.coli</i>:151
==August 17 (Fri)==
==August 17 (Fri)==
-
 
+
# Transfer to liquid culture
==August 18 (Sat)==
==August 18 (Sat)==
-
 
+
# Preparation of LB agar plates (Tet,Kan)
 +
# Miniprep of yesterday's culture:153~155
 +
# PCR
 +
#* mutation was found in PprA →　retrial
 +
# Purification of PCR products
 +
# Preparation of glycerol stock of cell culture:151
 +
# Restriction digests of minipreppped parts
 +
# Gel electrophoresis of digests
 +
#* 155 x
 +
# Ligation
 +
#* 156(T):153(upstream)+1-23L(downstream)+1-7A(vector)
 +
# Transformation of ligation products into DH5α <i>E.coli</i>:155,156
[[Team:Osaka/Notebook|Back to Notebook]]
[[Team:Osaka/Notebook|Back to Notebook]]

Latest revision as of 01:30, 14 September 2012


August 12 (Sun)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Ligation
    • 153(C):1-2M(upstream)+1-10H(downstream)+1-1A(vector)
    • 154(C):1-22A(upstream)+1-2M(downstream)+1-1A(vector)
    • 155(C):1-2D(upstream)+1-23L(downstream)+1-1A(vector)
  5. SDS-PAGE
    • Electrophoresis

August 16 (Thu)

  1. Transformaton of ligation products into DH5α E.coli:153~155
  2. Transformation of plasmid DNA into Rosetta E.coli:151

August 17 (Fri)

  1. Transfer to liquid culture

August 18 (Sat)

  1. Preparation of LB agar plates (Tet,Kan)
  2. Miniprep of yesterday's culture:153~155
  3. PCR
    • mutation was found in PprA →　retrial
  4. Purification of PCR products
  5. Preparation of glycerol stock of cell culture:151
  6. Restriction digests of minipreppped parts
  7. Gel electrophoresis of digests
    • 155 x
  8. Ligation
    • 156(T):153(upstream)+1-23L(downstream)+1-7A(vector)
  9. Transformation of ligation products into DH5α E.coli:155,156

Back to Notebook

Retrieved from "http://2012.igem.org/Team:Osaka/week3"