Team:Osaka/week2

From 2012.igem.org

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{{Osaka}}
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{{Osaka2}}
__NOTOC__
__NOTOC__
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==August 5 (Sun)==
==August 5 (Sun)==
# Miniprep of yesterday's culture
# Miniprep of yesterday's culture
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# Restriction digests of minipreppped parts
# Restriction digests of minipreppped parts
# Gel electrophoresis of digests  
# Gel electrophoresis of digests  
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# Transformaton of Registry parts into DH5α <i>E.coli</i>(See Table 2)
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# Transformation of Registry parts into DH5α <i>E.coli</i>(See Table 2)
# Preparation of glycerol stock of cell culture:143~146,149,152
# Preparation of glycerol stock of cell culture:143~146,149,152
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==August 8 (Wed)==
==August 8 (Wed)==
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# Transfer to liquid culture:1-2D,1-10H,1-22A
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# Transfer to liquid culture:1-2D,1-10H,1-22A,151
==August 9 (Thu)==
==August 9 (Thu)==
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==August 10 (Fri)==
==August 10 (Fri)==
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# Sequencing:143~152
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# Sequencing reaction:143~152
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# Transfer to liquid culture:1-2M
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# Sequencing purification
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# Preparation of glycerol stock of cell culture:147,148,150
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# Transfer to liquid culture:1-2M,147,148,150
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# Transformaton of Registry parts into DH5α <i>E.coli</i>:1-10H
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# Transformation of Registry parts into DH5α <i>E.coli</i>:1-10H
==August 11 (Sat)==
==August 11 (Sat)==
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# Miniprep of yesterday's culture
+
# Preparation of glycerol stock of cell culture:147,148,150
 +
# Miniprep of yesterday's culture:1-2M
# Restriction digests of minipreppped parts
# Restriction digests of minipreppped parts
# Transfer to liquid culture:1-10H
# Transfer to liquid culture:1-10H
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# SDS-PAGE  
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# SDS-PAGE :149,150,151,152,empty vector
#* IPTG induction
#* IPTG induction
[[Team:Osaka/Notebook|Back to Notebook]]
[[Team:Osaka/Notebook|Back to Notebook]]

Latest revision as of 01:30, 14 September 2012


August 5 (Sun)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Transfer to liquid culture
    • (Rosetta)143~146,149,152
    • (DH5α)150,Empty vector(A)

August 6 (Mon)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Transformation of Registry parts into DH5α E.coli(See Table 2)
  5. Preparation of glycerol stock of cell culture:143~146,149,152
Table2
IDPart NameResistanceDescription
1-2D<bbpart>BBa_J52008</bbpart>A,K Renilla Luciferase
1-10H<bbpart>BBa_I712019</bbpart>A,K Firefly Luciferase from Photinus pyralis
1-22A<bbpart>BBa_J23118</bbpart>A Constitutive promoter family member

August 7 (Tue)

  1. Preparation of LB agar plates (Cam)
  2. Transformation of 1-2D,1-10H

August 8 (Wed)

  1. Transfer to liquid culture:1-2D,1-10H,1-22A,151

August 9 (Thu)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • 1-10H not ok
  4. Transformaton of Registry parts into DH5α E.coli(See Table 3)
  5. Transformation of plasmid DNA into Rosetta E.coli:147,148,150
Table3
IDPart NameResistanceDescription
1-2M<bbpart>BBa_K118022</bbpart>ARBS

August 10 (Fri)

  1. Sequencing reaction:143~152
  2. Sequencing purification
  3. Transfer to liquid culture:1-2M,147,148,150
  4. Transformation of Registry parts into DH5α E.coli:1-10H

August 11 (Sat)

  1. Preparation of glycerol stock of cell culture:147,148,150
  2. Miniprep of yesterday's culture:1-2M
  3. Restriction digests of minipreppped parts
  4. Transfer to liquid culture:1-10H
  5. SDS-PAGE :149,150,151,152,empty vector
    • IPTG induction

Back to Notebook

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