Team:Osaka/week2

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Latest revision as of 01:30, 14 September 2012

August 5 (Sun)

Miniprep of yesterday's culture
Restriction digests of minipreppped parts
Gel electrophoresis of digests
Transfer to liquid culture
- (Rosetta)143~146,149,152
- (DH5α)150,Empty vector(A)

August 6 (Mon)

Miniprep of yesterday's culture
Restriction digests of minipreppped parts
Gel electrophoresis of digests
Transformation of Registry parts into DH5α E.coli(See Table 2)
Preparation of glycerol stock of cell culture:143~146,149,152

Table2
ID	Part Name	Resistance	Description
1-2D	<bbpart>BBa_J52008</bbpart>	A,K	Renilla Luciferase
1-10H	<bbpart>BBa_I712019</bbpart>	A,K	Firefly Luciferase from Photinus pyralis
1-22A	<bbpart>BBa_J23118</bbpart>	A	Constitutive promoter family member

August 7 (Tue)

Preparation of LB agar plates (Cam)
Transformation of 1-2D,1-10H

August 8 (Wed)

Transfer to liquid culture:1-2D,1-10H,1-22A,151

August 9 (Thu)

Miniprep of yesterday's culture
Restriction digests of minipreppped parts
Gel electrophoresis of digests
- 1-10H not ok
Transformaton of Registry parts into DH5α E.coli(See Table 3)
Transformation of plasmid DNA into Rosetta E.coli:147,148,150

Table3
ID	Part Name	Resistance	Description
1-2M	<bbpart>BBa_K118022</bbpart>	A	RBS

August 10 (Fri)

Sequencing reaction:143~152
Sequencing purification
Transfer to liquid culture:1-2M,147,148,150
Transformation of Registry parts into DH5α E.coli:1-10H

August 11 (Sat)

Preparation of glycerol stock of cell culture:147,148,150
Miniprep of yesterday's culture:1-2M
Restriction digests of minipreppped parts
Transfer to liquid culture:1-10H
SDS-PAGE :149,150,151,152,empty vector
- IPTG induction

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@@ Line 1: / Line 1: @@
-{{Osaka}}
+{{Osaka2}}
 __NOTOC__
 ==August 5 (Sun)==
 # Miniprep of yesterday's culture
@@ Line 13: / Line 14: @@
 # Restriction digests of minipreppped parts
 # Gel electrophoresis of digests
-# Transformaton of Registry parts into DH5α <i>E.coli</i>(See Table 2).
+# Transformation of Registry parts into DH5α <i>E.coli</i>(See Table 2)
+# Preparation of glycerol stock of cell culture:143~146,149,152
 {|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5"
 |+Table2
@@ Line 20: / Line 23: @@
 |1-2D||<bbpart>BBa_J52008</bbpart>||A,K|| Renilla Luciferase
 |-
-|1-10H||<bbpart>BBa_I712019</bbpart>||A,K|| Firefly Luciferase
+|1-10H||<bbpart>BBa_I712019</bbpart>||A,K|| Firefly Luciferase from Photinus pyralis
 |-
 |1-22A||<bbpart>BBa_J23118</bbpart>||A|| Constitutive promoter family member
 |}
 ==August 7 (Tue)==
+# Preparation of LB agar plates (Cam)
+# Transformation of 1-2D,1-10H
 ==August 8 (Wed)==
+# Transfer to liquid culture:1-2D,1-10H,1-22A,151
 ==August 9 (Thu)==
+# Miniprep of yesterday's culture
+# Restriction digests of minipreppped parts
+# Gel electrophoresis of digests
+#* 1-10H not ok
+# Transformaton of Registry parts into DH5α <i>E.coli</i>(See Table 3)
+# Transformation of plasmid DNA into Rosetta <i>E.coli</i>:147,148,150
+{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5"
+|+Table3
+!ID!!Part Name!!Resistance!!Description
+|-
+|1-2M||<bbpart>BBa_K118022</bbpart>||A||RBS
+|}
 ==August 10 (Fri)==
+# Sequencing reaction:143~152
+# Sequencing purification
+# Transfer to liquid culture:1-2M,147,148,150
+# Transformation of Registry parts into DH5α <i>E.coli</i>:1-10H
 ==August 11 (Sat)==
+# Preparation of glycerol stock of cell culture:147,148,150
+# Miniprep of yesterday's culture:1-2M
+# Restriction digests of minipreppped parts
+# Transfer to liquid culture:1-10H
+# SDS-PAGE :149,150,151,152,empty vector
+#* IPTG induction
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