Team:British Columbia/Interesting Biobricks

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Revision as of 20:17, 9 June 2012

Killswitch BioBricks
Numbers	Team	Year	Trigger	What it Does	Where?
<partinfo>BBa_I716462</partinfo>	UC-Berkeley	2007	Arabinose promoter	BamHI
<partinfo>BBa_K512002</partinfo>	USC	2011	IPTG, targets GFP gene	CRISPR-mediated GFP plasmid degradation
<partinfo>BBa_K112808</partinfo>	UC-Berkeley	2008	No Promoter	T4 Lysis device	Plate 3, 6G
<partinfo>BBa_K541545</partinfo>	FAGEM	2011	IPTG	Limulus Anti-Lipopolysaccharide Factor for E.coli (IPTG Inducible)
<partinfo>BBa_K593009</partinfo>	METU-Ankara	2011	T7 Promoter	ROSE regulated kill switch, (RNA thermometer, only activates above 42°)
<partinfo>BBa_K628006</partinfo>	St. Andrews	2011	pBad	Protegrin-1 Kill Switch, (Antimicrobial peptide)
	Strasbourg	2010		I don't know what the heck.
	Tokyo Metropolitan	2011		Killer Bee e.coli?
	UNIST Korea	2011		Killed by high and low temps, not if dark.
	Harvard	2010		Barnase cleaves RNA!
<partinfo>BBa_I716211</partinfo>	UC Berkeley	2007	No Promoter	Harvard got IPTG inducible Barnase/Barnstar from Anderson Lab at UCB
	Hong Kong	2010		Possible new killswitch, not implemented
<partinfo>BBa_K565001</partinfo>	Valencia	2011	? 12 kb	Colicin G
<partinfo>BBa_K565002</partinfo>	Valencia	2011	? 12 kb	Colicin H
<partinfo>BBa_K565004</partinfo>	Valencia	2011	No Promoter	Microcin C51 production device (5 restriction sites)
<partinfo>BBa_K185004</partinfo>	SJTU-BioX-Shanghai	2009	No Promoter	RelE toxin+Double terminator
	UNICAMP Brazil	2009		All sorts of kill switches, but may not all work in E. coli.
<partinfo>BBa_K284017</partinfo>	UNICAMP-Brazil	2009	Lactate (Yeast)	Lysozyme
<partinfo>BBa_K131000</partinfo>	Calgary	2008	SOS Promoter	Colicin 2E	3, 22A
<partinfo>BBa_K112806</partinfo>	UC Berkeley	2008	T7	T4 Endolysin
<partinfo>BBa_K302035</partinfo>	Newcastle	2010	Sucrose limit	MazEF
<partinfo>BBa_K124014</partinfo>	Brown	2008	No Promoter	Mutated Holin Gene (Inconsistent sequencing)
<partinfo>BBa_K419003</partinfo>	TzuChiU_Formosa	2010	pLys	Lysis Cassette - no antiholin
<partinfo>BBa_K317039</partinfo>	Tokyo-NoKoGen	2010	AHL	AHL sensitive lysis cassette
<partinfo>BBa_K317040</partinfo>	Tokyo-NoKoGen	2010	IPTG	IPTG sensitive lysis cassette
<partinfo>BBa_K299806</partinfo>	Warsaw	2010		MinC cell division inhibitor
<partinfo>BBa_K150007</partinfo>	Heidelberg	2008	Native Operon	Colicin E1
<partinfo>BBa_K150010</partinfo>	Heidelberg	2008	Native Operon	Colicin E9
<partinfo>BBa_K145230</partinfo>	KULeuven	2008	HSL-LuxR	ccdb
<partinfo>BBa_K117009</partinfo>	NTU-Singapore	2008	IPTG	Colicin E7
<partinfo>BBa_K117000</partinfo>	NTU-Singapore	2008		Lysin (activates Colicin E7)
<partinfo>BBa_K108001</partinfo>	Tsinghua	2008		Enterobacter phage lysis

Distribution BioBricks
Location	Part Name	Function	Use	Other	Plasmid Resistance
Plate 1
5P	<partinfo>BBa_P1003</partinfo>	Kan Resistance	Resistance	Full Cassette	"Kan	Amp	Tet"
7B	<partinfo>BBa_P1004</partinfo>	Chlor Resist	Resistance	Full Cassette	"Kan	Amp"
14C	<partinfo>BBa_C0261</partinfo>	AHL Synthase	AHL Kill Switch	No Promoter	Amp
24E	<partinfo>BBa_I13600</partinfo>	CFP reporter	fluorescence	0	Amp
Plate 2
17F	<partinfo>BBa_I20260</partinfo>	GFP reporter	Fluorescence	Strong const.	Kan
14N	<partinfo>BBa_K117009</partinfo>	Colicin E7	Kill Switch	Lactose prom	Amp
14D	<partinfo>BBa_K117000</partinfo>	Lysis gene	Kill Switch	Aids colicin	Amp
9B	<partinfo>BBa_J09250</partinfo>	GFP reporter	Fluoresence	Strong Const.	Kan
Plate 3
2D	<partinfo>BBa_I746909</partinfo>	Superfold GFP	Fluoresence	T7	Amp
5G	<partinfo>BBa_K137076</partinfo>	H2O2 gen.	Kill Switch	AHL induce	Amp
6G	<partinfo>BBa_K112808</partinfo>	Lysis Device	Kill Switch	No Promoter	Amp
13M	<partinfo>BBa_K093012</partinfo>	RFP	Fluorescence	Strong const.	Amp
14E	<partinfo>BBa_K081018</partinfo>	TetR	Resistance	Strong const.	"Amp	Kan"
15B	<partinfo>BBa_K112000</partinfo>	Holin	Kill Switch	No promoter	?Spec
15D	<partinfo>BBa_K112005</partinfo>	"RBS, Holin"	Kill Switch	No Promoter	?Spec
15H	<partinfo>BBa_k112017</partinfo>	lysozyme	Kill Switch	No Promoter	?Spec
15J	<partinfo>BBa_K112106</partinfo>	BgIII endonuc.	Kill Switch	No Promoter	?Spec
20I	<partinfo>BBa_K124003</partinfo>	Lysis Cassette	Kill Switch	?	Kan
20K	<partinfo>BBa_K124014</partinfo>	M S Holin	Kill Switch	No Promoter	"Amp	Kan"
20M	<partinfo>BBa_K124017</partinfo>	M Lysis cassette	Kill Switch	No Promoter	Kan
21O	<partinfo>BBa_K106674</partinfo>	GFP	Fluorescence	Med. Const.	?amp
22A	<partinfo>BBa_K131000</partinfo>	Colicin E2	Kill Switch	SOS promoter	"Kan	Amp"
22C	<partinfo>BBa_K131010</partinfo>	Colicin E2	Kill Switch	"AHL induce, c GFP"	"Kan	Amp"
24E	<partinfo>BBa_K112022</partinfo>	Lysis Device	Kill Switch	"No Prom, weak anti protein"	"?Amp	Kan"
Plate 4
3I	<partinfo>BBa_K398406</partinfo>	Solvent Tolerant	Other (Hex. Tol)	Strong const.	Chlor
6M	<partinfo>BBa_K112806</partinfo>	T4 Endolysin	Kill Switch	No Prom	Amp
7C	<partinfo>BBa_K314200</partinfo>	Tse Toxin	Kill Switch	No Prom	Chlor
8G	<partinfo>BBa_K206000</partinfo>	pBAD Promoter	Promoter	Arabinose ind	Amp
21G	<partinfo>BBa_K314203</partinfo>	Tse Tox. Ind.Anti	Kill switch	HSL ind immunity	Chlor
Plate 5
1A	<partinfo>BBa_K515107</partinfo>	Photo-Switch	Death test/Fluor	pTet	Chlor
12O	<partinfo>BBa_I13604</partinfo>	Con. YFP ind CFP	Fluor	Strong const./lacI	Amp
14I	<partinfo>BBa_K299806</partinfo>	minC	Kill Switch	No Promoter	Amp

@@ Line 1: / Line 1: @@
 {| border="1"
 |+ Killswitch BioBricks
-! scope="col" | Numbers
+! Numbers !! Team !! Year !! Trigger !! What it Does !! Where?
-! scope="col" | Team
-! scope="col" | Year
-! scope="col" | Trigger
-! scope="col" | What it Does
-! scope="col" | Where?
 |-
 | <partinfo>BBa_I716462</partinfo> ||UC-Berkeley ||2007|| Arabinose promoter || BamHI ||On Plate 2, 3C|