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Team:Wageningen UR/Methods
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The use of Virus-Like-Particles as medicine carrier is new for iGEM. This means the whole production, purification and detection of Virus-Like-Particles is also new in iGEM. In this section we will explain how the different methods work and how it all fits together. | The use of Virus-Like-Particles as medicine carrier is new for iGEM. This means the whole production, purification and detection of Virus-Like-Particles is also new in iGEM. In this section we will explain how the different methods work and how it all fits together. | ||
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The production of our VLPs monomers is done in E.coli, while the formation of our VLPs is done in vitro. We used multiple techniques to make this happen. [[Team:Wageningen_UR/MethodsProduction|Click here to read more]] | The production of our VLPs monomers is done in E.coli, while the formation of our VLPs is done in vitro. We used multiple techniques to make this happen. [[Team:Wageningen_UR/MethodsProduction|Click here to read more]] | ||
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After the production of our VLPs we have our formed VLPs, but this product is not pure enough. So we developed a two step method to purify the product. [[Team:Wageningen_UR/MethodsPurification|Click here to read more]] | After the production of our VLPs we have our formed VLPs, but this product is not pure enough. So we developed a two step method to purify the product. [[Team:Wageningen_UR/MethodsPurification|Click here to read more]] | ||
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The most difficult and possible the most expensive thing is the detection of our VLPs. We found multiple methods that can help us. [[Team:Wageningen_UR/MethodsDetection|Click here to read more]] | The most difficult and possible the most expensive thing is the detection of our VLPs. We found multiple methods that can help us. [[Team:Wageningen_UR/MethodsDetection|Click here to read more]] | ||
Revision as of 09:12, 13 September 2012
General Overview
The use of Virus-Like-Particles as medicine carrier is new for iGEM. This means the whole production, purification and detection of Virus-Like-Particles is also new in iGEM. In this section we will explain how the different methods work and how it all fits together.
The production of our VLPs monomers is done in E.coli, while the formation of our VLPs is done in vitro. We used multiple techniques to make this happen. Click here to read more
After the production of our VLPs we have our formed VLPs, but this product is not pure enough. So we developed a two step method to purify the product. Click here to read more
The most difficult and possible the most expensive thing is the detection of our VLPs. We found multiple methods that can help us. Click here to read more
