Team:Wageningen UR/Methods

From 2012.igem.org

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(General Overview)
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The use of Virus-Like-Particles is new for iGEM and so is the methods that lead to VLP formation. So we put here a simple overview on how the methods work and why we would use it.
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The use of Virus-Like-Particles as medicine carrier is new for iGEM. This means the whole production, purification and detection of Virus-Like-Particles is also new in iGEM. In this section we will explain how the different methods work and how it all fits together.
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<li>[[Team:Wageningen_UR/MethodsProduction|Production of VLPs]]
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<li>[[Team:Wageningen_UR/MethodsPurification|Purification of VLPs]]
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The production of our VLPs monomers is done in E.coli, while the formation of our VLPs is done in vitro. We used multiple techniques to make this happen. [[Team:Wageningen_UR/MethodsProduction|Click here to read more]]
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<li>[[Team:Wageningen_UR/MethodsDetection|Detection of VLPs]]
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After the production of our VLPs we have our formed VLPs, but this product is not pure enough. So we developed a two step method to purify the product. [[Team:Wageningen_UR/MethodsPurification|Click here to read more]]
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The most difficult and possible the most expensive thing is the detection of our VLPs. We found multiple methods that can help us. [[Team:Wageningen_UR/MethodsDetection|Click here to read more]]

Revision as of 09:12, 13 September 2012

General Overview

The use of Virus-Like-Particles as medicine carrier is new for iGEM. This means the whole production, purification and detection of Virus-Like-Particles is also new in iGEM. In this section we will explain how the different methods work and how it all fits together.

The production of our VLPs monomers is done in E.coli, while the formation of our VLPs is done in vitro. We used multiple techniques to make this happen. Click here to read more

After the production of our VLPs we have our formed VLPs, but this product is not pure enough. So we developed a two step method to purify the product. Click here to read more

The most difficult and possible the most expensive thing is the detection of our VLPs. We found multiple methods that can help us. Click here to read more