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Team:SDU-Denmark/labwork/Protocols/PCR
From 2012.igem.org
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<img src="https://static.igem.org/mediawiki/2012/b/b4/Hot_Start.png" alt="some_text"/> | <img src="https://static.igem.org/mediawiki/2012/b/b4/Hot_Start.png" alt="some_text"/> | ||
| - | + | </br> | |
| + | </br> | ||
| + | PCR program: </br> | ||
| + | <pre>95°C for 3 minutes | ||
| + | <pre>30 cycles | ||
| + | 95°C for 30 seconds | ||
| + | Annealing temperature (5°C below primer melting temperature) | ||
| + | 59°C for 20 seconds</pre>72°C for 3 minutes</pre> | ||
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Revision as of 13:12, 12 September 2012
| mRNA Isolation | PCR | Miniprep | Check Digest |
| Content | Content | Content | Content |
| Content | Content | Content | Content |
Polymerase Chain Reaction
Proof-reading PCR using Phusion Hot Start II
PCR program:
95°C for 3 minutes30 cycles 95°C for 30 seconds Annealing temperature (5°C below primer melting temperature) 59°C for 20 seconds72°C for 3 minutes
