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Team:Wageningen UR/Journal/week14
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* The four 'brickable' TuYV CP gene amplicons were digested, ligated into the backbone from BBa_J04450 and transformed into our electrocompetent Mach1 ''E coli''. Despite negative results on two PCR checks, minipreps were done anyway and a third PCR check showed presence of all of our inserts in their respective minipreps! | * The four 'brickable' TuYV CP gene amplicons were digested, ligated into the backbone from BBa_J04450 and transformed into our electrocompetent Mach1 ''E coli''. Despite negative results on two PCR checks, minipreps were done anyway and a third PCR check showed presence of all of our inserts in their respective minipreps! | ||
| + | '''PLRV''' | ||
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| + | The PCR products obtained after Reverse Transcription followed by PCR we used as template to add iGEM prefix and suffix. | ||
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[[https://2012.igem.org/Team:Wageningen_UR/Journal/week13 previous week]] [[https://2012.igem.org/Team:Wageningen_UR/Journal/week15 next week]] | [[https://2012.igem.org/Team:Wageningen_UR/Journal/week13 previous week]] [[https://2012.igem.org/Team:Wageningen_UR/Journal/week15 next week]] | ||
Revision as of 09:40, 12 September 2012
week 14: 30 july - 5 august
Lab work
Bricking Hepatitis B
Monday:
- Miniprepping
of 8 colonies from the transformation (Hep B + BBa_J04500)
- Digestion check
of those minipreps
Wednesday:
- Second digestion check of the minipreps (with higher amount of DNA)
the digestion check confirmed the right insert sizes in the samples they where expected (slots 3,4,10,11)
TuYV
- The four 'brickable' TuYV CP gene amplicons were digested, ligated into the backbone from BBa_J04450 and transformed into our electrocompetent Mach1 E coli. Despite negative results on two PCR checks, minipreps were done anyway and a third PCR check showed presence of all of our inserts in their respective minipreps!
PLRV
The PCR products obtained after Reverse Transcription followed by PCR we used as template to add iGEM prefix and suffix.
