From 2012.igem.org

Revision as of 09:36, 12 September 2012

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  • 1. Introduction
  • 2. PnA System
  • 3. Virus-Like Particles
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    • Polerovirus Natural BioBrick
  • 4. Outside Modification
  • 5. Inside Modification
  • 6. Detection of VLPs
  • 7. Applications
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• Human Practices
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  • 1. Mini Symposium
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  • 1. General safety
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  • 4. Safety suggestions
  • 5. Safety of applications
• Modeling
  • Human Body Model
  • Tertiary folding prediction
  • VLP assembly model
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Last year's project: Synchronized Oscillatory System

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week 13: 23 july - 29 july

Not much work was done this week because the team spent four days on going to a three day synthetic biology conference in Munich. We learned a lot there and had a great time!

Office work

Lab work

PLRV

• RNA isolation of potato leaf tissue

• Reverse transcriptase reaction to obtain cDNA

• PCR on cDNA using coat protein primers

The figure below shows the purity of isolated RNA. We isolated RNA of different potato cultivars.

We performed Reverse Transciptase reactions followed by PCR. We used the Revert AID H Minus Reverse Transcription Kit (Fermentas).

Agarose gel of PCR products. Lanes 1 till 4 show the PLRV Coat Protein (CP) bands with the expected size of 627 bp. Lanes 6 till 9 show the PLRV Coat Protein plus Read Through part (CP_RT) with the expected size of 2154 bp.

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