"
Team:TU Darmstadt/Materials/SpeI
From 2012.igem.org
(Difference between revisions)
(Created page with "<html> <link rel="stylesheet" href="https://2012.igem.org/wiki/index.php?title=Team:TU_Darmstadt/css&action=raw&ctype=text/css" type="text/css" /> <div id="TUD"> <div id="...") |
|||
| Line 49: | Line 49: | ||
'''Buffer:''' NEBuffer 2 + BSA | '''Buffer:''' NEBuffer 2 + BSA | ||
| - | SpeI requires at least 2bp overhang on its cutting site (100% cleavage efficiency).<sup>[ | + | SpeI requires at least 2bp overhang on its cutting site (100% cleavage efficiency).<sup>[2]</sup> |
=== Supplier === | === Supplier === | ||
* NEB http://www.neb.com/nebecomm/products/productR3133.asp | * NEB http://www.neb.com/nebecomm/products/productR3133.asp | ||
Revision as of 19:01, 11 September 2012
Contents |
SpeI
About
SpeI is a endonucleasic restriction enzyme isolated from Sphaerotilus natans. It creates stick ends with 5' end overhang.
Working conditions
The recognition motive of SpeI is:[1]
5' ACTAGT --> 5'---A CTAGT---3' 3' TGATCA --> 3'---TGATC A---5'
Buffer: NEBuffer 2 + BSA
SpeI requires at least 2bp overhang on its cutting site (100% cleavage efficiency).[2]
Supplier
- NEB http://www.neb.com/nebecomm/products/productR3133.asp
References
[1] http://en.wikipedia.org/wiki/List_of_restriction_enzyme_cutting_sites:_S#Whole_list_navigation
[2] http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_linearized_vector.asp
