Team:Wageningen UR/Protocol

From 2012.igem.org

(Difference between revisions)
(→General Protocol)
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== Medium & Buffer recipes ==
== Medium & Buffer recipes ==
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*[[Team:Wageningen_UR/Protocol/LBmedium|LB medium]]
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<ul>
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*[[Team:Wageningen_UR/Protocol/SOBmedium|SOB medium]]
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<li>[[Team:Wageningen_UR/Protocol/LBmedium|LB medium]]</li>
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*[[Team:Wageningen_UR/Protocol/SOCmedium|SOC medium]]
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<li>[[Team:Wageningen_UR/Protocol/SOBmedium|SOB medium]]</li>
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*[[Team:Wageningen_UR/Protocol/Disassemblybuffer|Disassembly buffer]]
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<li>[[Team:Wageningen_UR/Protocol/SOCmedium|SOC medium]]</li>
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*[[Team:Wageningen_UR/Protocol/Dialysisbuffer10x|Dialysis buffer 10x]]
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<li>[[Team:Wageningen_UR/Protocol/Disassemblybuffer|Disassembly buffer]]</li>
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*[[Team:Wageningen_UR/Protocol/Reassemblybuffer|Reassembly buffer]]
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<li>[[Team:Wageningen_UR/Protocol/Dialysisbuffer10x|Dialysis buffer 10x]]</li>
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*[[Team:Wageningen_UR/Protocol/Virusbuffer|Virus buffer]]
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<li>[[Team:Wageningen_UR/Protocol/Reassemblybuffer|Reassembly buffer]]</li>
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*[[Team:Wageningen_UR/Protocol/Towbinselectrotransferbuffer1x|Towbin's electrotransfer buffer 1x]]
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<li>[[Team:Wageningen_UR/Protocol/Virusbuffer|Virus buffer]]</li>
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*[[Team:Wageningen_UR/Protocol/Towbinselectrotransferbuffer10x|Towbin's electrotransfer buffer 10x]]
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<li>[[Team:Wageningen_UR/Protocol/Towbinselectrotransferbuffer1x|Towbin's electrotransfer buffer 1x]]</li>
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<li>[[Team:Wageningen_UR/Protocol/Towbinselectrotransferbuffer10x|Towbin's electrotransfer buffer 10x]]</li>
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</ul>
== CCMV Coat Protein VLP formation ==
== CCMV Coat Protein VLP formation ==
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*[[Team:Wageningen_UR/Protocol/StartupCCMV|Growing culture]]
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<ul>
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*[[Team:Wageningen_UR/Protocol/DialysisCCMV|Dialysis of the VLPs]]
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<li>[[Team:Wageningen_UR/Protocol/StartupCCMV|Growing culture]]</li>
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*[[Team:Wageningen_UR/Protocol/RoundupCCMV|Purifing the VLPs]]
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<li>[[Team:Wageningen_UR/Protocol/DialysisCCMV|Dialysis of the VLPs]]</li>
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<li>[[Team:Wageningen_UR/Protocol/RoundupCCMV|Purifing the VLPs]]</li>
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</ul>
== Hepatitis B Coat Protein VLP formation ==
== Hepatitis B Coat Protein VLP formation ==
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== PLRV Coat Protein VLP formation ==
== PLRV Coat Protein VLP formation ==
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*[[Team:Wageningen_UR/Protocol/RNA|RNA isolation from potato leaf material]]
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<ul>
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<li>[Team:Wageningen_UR/Protocol/RNA|RNA isolation from potato leaf material]]</li>
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</ul>
== General Protocol ==
== General Protocol ==
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*[[Team:Wageningen_UR/Protocol/ElectrocompetentCells|Preparation of electrocompetent cells of ''E. coli'']]
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<ul>
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*[[Team:Wageningen_UR/Protocol/SDSPolyacrylamide|SDS-Polyacrylamide and native gel electrophoresis]]
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<li>[[Team:Wageningen_UR/Protocol/ElectrocompetentCells|Preparation of electrocompetent cells of ''E. coli'']]</li>
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*[[Team:Wageningen_UR/Protocol/DynamicLightScatteringMachine|Dynamic Light Scattering user manual]]
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<li>[[Team:Wageningen_UR/Protocol/SDSPolyacrylamide|SDS-Polyacrylamide and native gel electrophoresis]]</li>
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*[[Team:Wageningen_UR/Protocol/Frenchpress|French press user manual]]
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<li>[[Team:Wageningen_UR/Protocol/DynamicLightScatteringMachine|Dynamic Light Scattering user manual]]</li>
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*[[Team:Wageningen_UR/Protocol/FPLC|FPLC user manual]]
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<li>[[Team:Wageningen_UR/Protocol/Frenchpress|French press user manual]]</li>
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<li>[[Team:Wageningen_UR/Protocol/FPLC|FPLC user manual]]</li>
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</ul>

Revision as of 11:23, 10 September 2012

Contents

Protocol

Medium & Buffer recipes

CCMV Coat Protein VLP formation

Hepatitis B Coat Protein VLP formation

TuYV Coat Protein VLP formation

PLRV Coat Protein VLP formation

  • [Team:Wageningen_UR/Protocol/RNA|RNA isolation from potato leaf material]]

General Protocol

Retrieved from "http://2012.igem.org/Team:Wageningen_UR/Protocol"