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Team:TU Darmstadt/Materials/DYT
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Ingredients for 1.7- DYT medium | Ingredients for 1.7- DYT medium | ||
| - | For 500 mL | + | '''For 500 mL''' |
* 2.5 g NaCl | * 2.5 g NaCl | ||
* 8.0 g tryptone | * 8.0 g tryptone | ||
* 5.0 g yeast extract | * 5.0 g yeast extract | ||
| - | For 100 mL | + | '''For 100 mL''' |
* 0.5 g NaCl | * 0.5 g NaCl | ||
* 1.6 g tryptone | * 1.6 g tryptone | ||
* 1.0 g yeast extract | * 1.0 g yeast extract | ||
| - | Continue to: | + | '''Continue to:''' |
*mix in ddH<sub>2</sub>O | *mix in ddH<sub>2</sub>O | ||
*add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough) | *add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough) | ||
Revision as of 23:16, 9 September 2012
DYT
Ingredients for 1.7- DYT medium
For 500 mL
- 2.5 g NaCl
- 8.0 g tryptone
- 5.0 g yeast extract
For 100 mL
- 0.5 g NaCl
- 1.6 g tryptone
- 1.0 g yeast extract
Continue to:
- mix in ddH2O
- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)
- aliquot: add 3 ml to 15 ml tubes (about two thumbs high) cap loosely
- autoclave in a rack in a tub with at least 4 cm of water
- store at 4°C
