Team:NCTU Formosa/Project
From 2012.igem.org
Line 6: | Line 6: | ||
<p id="back-top"><a href="#top"><span></span>Back to Top</a></p> | <p id="back-top"><a href="#top"><span></span>Back to Top</a></p> | ||
<nav id="submenu"> | <nav id="submenu"> | ||
- | <div class="subst1"> | + | <div id="submenu1" class="subst1"> |
<h1><a href="#sub1">Introduction</a></h1> | <h1><a href="#sub1">Introduction</a></h1> | ||
<p>Introduction</p> | <p>Introduction</p> | ||
</div> | </div> | ||
- | <div class="subst2"> | + | <div id="submenu2-1" class="subst2"> |
<h1><a href="#sub2-1">Enzyme for Isobutanol</a></h1> | <h1><a href="#sub2-1">Enzyme for Isobutanol</a></h1> | ||
<p>Enzyme for Isobutanol</p> | <p>Enzyme for Isobutanol</p> | ||
</div> | </div> | ||
- | <div class="subst2"> | + | <div id="submenu2-2" class="subst2"> |
<h1><a href="#sub2-2">Temperature Control System</a></h1> | <h1><a href="#sub2-2">Temperature Control System</a></h1> | ||
<p>Temperature Control System</p> | <p>Temperature Control System</p> | ||
</div> | </div> | ||
- | <div class="subst2"> | + | <div id="submenu2-3" class="subst2"> |
<h1><a href="#sub2-3">Zinc Finger</a></h1> | <h1><a href="#sub2-3">Zinc Finger</a></h1> | ||
<p>Zinc Finger</p> | <p>Zinc Finger</p> | ||
</div> | </div> | ||
- | <div class="subst2"> | + | <div id="submenu2-4" class="subst2"> |
<h1><a href="#sub2-4">Instrument</a></h1> | <h1><a href="#sub2-4">Instrument</a></h1> | ||
<p>Instrument</p> | <p>Instrument</p> | ||
</div> | </div> | ||
- | <div class="subst1"> | + | <div id="submenu3" class="subst1"> |
<h1><a href="#sub3">Conclusion</a></h1> | <h1><a href="#sub3">Conclusion</a></h1> | ||
<p>Conclusion</p> | <p>Conclusion</p> |
Revision as of 15:47, 9 September 2012
Project details
Enzyme for isobutanol
(developing)
Temperature control system
The low temperature release system is a way to let e.coli produce isobutanol efficiently . Because isobutanol and isobutyaldehyde are toxic to the e.coli , the system avoid e.coli facing them at the beginning . The following picture is our system.
At the beginning , we will let E.coli stay in 37°C environment. After having enough 2-Ketoisovalerate , we will move E.coli into 30°C environment for producing the final product , isobutanol. It can make us get isobutanol successfully and efficiently.
This is our biobrick. The most important part of our biobrick is 37°C ribosome binding site gene. We separate our biobrick into two parts . The first part is which has 37℃ ribosome binding site gene and the second part is under the first one.
And now we're introducing how our system works.
When being in 37°C environment, the first part will be translated and produce tetR protein to inhibit Ptet promoter. The second part will not be translated. Then we can produce intermediate , 2-Ketoisovalerate.
After getting enough 2-Ketoisovalerate , E.coli will stay in 30°C environment. The ribosome will not bind the 37°C ribosome binding site and tetR genes will not be translated. Then the second part will be translated successfully. At the end , we can get the isobutanol.
Result
The report shows that we use fluorescene protein to mark the second part of our biobrick . We can see that fluorescene protein staying in 30°C environment will have higher expression than staying in 37°C environment . According to it , we can see that our system do truly work!
Zinc finger
(developing)
Result
(developing)
Instrument
(developing)