Team:NCTU Formosa/Project
From 2012.igem.org
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- | <p id="back-top"> | + | <p id="back-top" class="subst1"><a href="#top"><span></span>Back to Top</a></p> |
- | + | <p id="submenu1" class="subst1"><a href="#top"><span></span>Introduction</a></p> | |
- | + | <p id="submenu2-1" class="subst2"><a href="#top"><span></span>Enzyme for Isobutanol</a></p> | |
+ | <p id="submenu2-2" class="subst2"><a href="#top"><span></span>Temperature Control System</a></p> | ||
+ | <p id="submenu2-3" class="subst2"><a href="#top"><span></span>Zinc Finger</a></p> | ||
+ | <p id="submenu2-4" class="subst2"><a href="#top"><span></span>Instrument</a></p> | ||
+ | <p id="submenu3" class="subst1"><a href="#top"><span></span>Conclusion</a></p> | ||
<div id="project-s1-wrapper" class="project-s-wrapper"> | <div id="project-s1-wrapper" class="project-s-wrapper"> | ||
<div id="project-s1" class="project-s"> | <div id="project-s1" class="project-s"> | ||
- | <h1 id="project-s1-title" class="project-s-title"> <span>Introduction to the project</span></h1> | + | <h1 id="project-s1-title" class="project-s-title"><a name="sub1"> </a> <span>Introduction to the project</span></h1> |
<p>(developing)</p></div> | <p>(developing)</p></div> | ||
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<div id="project-s2-wrapper" class="project-s-wrapper"> | <div id="project-s2-wrapper" class="project-s-wrapper"> | ||
<div id="project-s2" class="project-s"> | <div id="project-s2" class="project-s"> | ||
- | <h1 id="project-s2-title" class="project-s-title"> <span>Project details</span></h1> | + | <h1 id="project-s2-title" class="project-s-title"><a name="sub2"> </a> <span>Project details</span></h1> |
- | <h2 id="project-s2-1-title" class="project-s-title"> <span>Enzyme for isobutanol</span></h2> | + | <h2 id="project-s2-1-title" class="project-s-title"><a name="sub2-1"> </a> <span>Enzyme for isobutanol</span></h2> |
<p>(developing)</p> | <p>(developing)</p> | ||
- | <h2 id="project-s2-2-title" class="project-s-title"> <span>Temperature control system</span></h2> | + | <h2 id="project-s2-2-title" class="project-s-title"><a name="sub2-2"> </a> <span>Temperature control system</span></h2> |
<p>The low temperature release system is a way to let e.coli produce isobutanol efficiently . Because isobutanol and isobutyaldehyde are toxic to the e.coli , the system avoid e.coli facing them at the beginning . The following picture is our system.</p> | <p>The low temperature release system is a way to let e.coli produce isobutanol efficiently . Because isobutanol and isobutyaldehyde are toxic to the e.coli , the system avoid e.coli facing them at the beginning . The following picture is our system.</p> | ||
<div id="project-s2-2-p1" class="pimg"> </div> | <div id="project-s2-2-p1" class="pimg"> </div> | ||
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<div id="project-s2-2-p5" class="pimg"> </div> | <div id="project-s2-2-p5" class="pimg"> </div> | ||
<p>The report shows that we use fluorescene protein to mark the second part of our biobrick . We can see that fluorescene protein staying in 30°C environment will have higher expression than staying in 37°C environment . According to it , we can see that our system do truly work!</p> | <p>The report shows that we use fluorescene protein to mark the second part of our biobrick . We can see that fluorescene protein staying in 30°C environment will have higher expression than staying in 37°C environment . According to it , we can see that our system do truly work!</p> | ||
- | <h2 id="project-s2-3-title" class="project-s-title"> <span>Zinc finger</span></h2> | + | <h2 id="project-s2-3-title" class="project-s-title"><a name="sub2-3"> </a> <span>Zinc finger</span></h2> |
<p>(developing)</p> | <p>(developing)</p> | ||
<h2 id="project-s2-s-title" class="project-s-title"> <span>Result</span></h2> | <h2 id="project-s2-s-title" class="project-s-title"> <span>Result</span></h2> | ||
<p>(developing)</p> | <p>(developing)</p> | ||
- | <h2 id="project-s2-4-title" class="project-s-title"> <span>Instrument</span></h2> | + | <h2 id="project-s2-4-title" class="project-s-title"><a name="sub2-4"> </a> <span>Instrument</span></h2> |
<p>(developing)</p></div> | <p>(developing)</p></div> | ||
</div> | </div> | ||
<div id="project-s3-wrapper" class="project-s-wrapper"> | <div id="project-s3-wrapper" class="project-s-wrapper"> | ||
<div id="project-s3" class="project-s"> | <div id="project-s3" class="project-s"> | ||
- | <h1 id="project-s3-title" class="project-s-title"> <span>Conclusion</span></h1> | + | <h1 id="project-s3-title" class="project-s-title"><a name="sub3"> </a> <span>Conclusion</span></h1> |
<p>(developing)</p></div> | <p>(developing)</p></div> | ||
</div> | </div> |
Revision as of 16:10, 8 September 2012
Project details
Enzyme for isobutanol
(developing)
Temperature control system
The low temperature release system is a way to let e.coli produce isobutanol efficiently . Because isobutanol and isobutyaldehyde are toxic to the e.coli , the system avoid e.coli facing them at the beginning . The following picture is our system.
At the beginning , we will let E.coli stay in 37°C environment. After having enough 2-Ketoisovalerate , we will move E.coli into 30°C environment for producing the final product , isobutanol. It can make us get isobutanol successfully and efficiently.
This is our biobrick. The most important part of our biobrick is 37°C ribosome binding site gene. We separate our biobrick into two parts . The first part is which has 37℃ ribosome binding site gene and the second part is under the first one.
And now we're introducing how our system works.
When being in 37°C environment, the first part will be translated and produce tetR protein to inhibit Ptet promoter. The second part will not be translated. Then we can produce intermediate , 2-Ketoisovalerate.
After getting enough 2-Ketoisovalerate , E.coli will stay in 30°C environment. The ribosome will not bind the 37°C ribosome binding site and tetR genes will not be translated. Then the second part will be translated successfully. At the end , we can get the isobutanol.
Result
The report shows that we use fluorescene protein to mark the second part of our biobrick . We can see that fluorescene protein staying in 30°C environment will have higher expression than staying in 37°C environment . According to it , we can see that our system do truly work!
Zinc finger
(developing)
Result
(developing)
Instrument
(developing)