Team:TU Darmstadt/Materials/XbaI

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== XbaI ==
=== About ===
XbaI is a endonucleasic [https://2012.igem.org/Team:TU_Darmstadt/Materials#Restriction_enzymes restriction enzyme] isolated from ''[http://en.wikipedia.org/wiki/Xanthomonas_badrii Xanthomonas badrii]''. It creates stick ends with 5' end overhang.

=== Working conditions ===
The recognition motive of XbaI is:<sup>[1]</sup>
 5' TCTAGA --> 5'---T     CTAGA---3'
 3' AGATCT --> 3'---AGATC     T---5'
'''Buffer:''' NEBuffer 2 + BSA

SpeI requires at least 1bp overhang on its cutting site (99% cleavage efficiency).<sup>[2]</sup>
=== Supplier ===
* NEB http://www.neb.com/nebecomm/products/productR0145.asp
=== References ===
[1] http://en.wikipedia.org/wiki/List_of_restriction_enzyme_cutting_sites:_T-Z#X

[2] http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_linearized_vector.asp