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User:Manux
From 2012.igem.org
Cyanide is considered an extremely harmful toxic for the enviroment and living organisms since it inhibits the celullar respiration at the level of electron transport chain. It is also very common in the mining industry as a way to recover metals. Due to its application and toxicity, it is necessary to monitor and keep the cyanide at subtoxic level. In this proyect we are generating a new alternative by using genetic engineering to modify E. coli genetically.
We will incorporate genes that will allow the bacteria to become a biosensor with the capacity to detect the presence of cyanide and cyanide compunds by adding the expression of a reporter gene (RFP) under the control of a promoter inducible by these compunds.
This gene comes from the bacteria ''Pseudomonas pseudoalcaligenes''. This new technique wich will be used to detect water and soil, will also become a platform so that in the future we could incorporate a gene that allows the bacteria, not only detect, but also degrade these compunds using a method that is accesible and environmentally friendly through bioremediation. consectetuer adipiscing elit. Mauris fermentum dictum magna. Sed laoreet aliquam leo.
Genetically Modified ''E. coli'' as an Alternative Biosensor of Cyanide and Cyanide Compunds
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