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From 2012.igem.org

I. Have Jason Repeat Experiment from Saturday with 10, 20, and 30 ug/mL Tetracycline Plates

II. Design Primers for pBAV1K to Check Orientation of Beta Homolog Library

• Order primers from Keck with the following names/sequences:

Name	Tm	Length (bp)	Sequence
pTest_F	63.88	35	5'-CGGTGATATTCTCATTTTAGCCATTTATTATTTCC-3'
pTest_R	65.48	28	5'-GAGCCTTTCGTTTTATTTGATGCCTCTG-3'

III. Inoculate Overnight Liquid Cultures of WT ADP1

• Select 4 colonies from ADP1 plate and inoculate 1 each into 2 mL of LB
• Incubate with shaking overnight at 34 ^oC