Team:ZJU-China/n p 3.htm
From 2012.igem.org
3 Cell transformation
3.1 Remove competent cells on ice to thaw.
3.2 Add 1-10 μl of DNA to the cells.
3.3 Incubate on ice for 20 min.
3.4 Spread onto LB + antibiotic agar plate. Incubate overnight at 37℃.