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From 2012.igem.org

1. In preparation, the night before, a colony was picked into 10ml of LB plus 10ul CMP

2. After, five dilutions were made from the sample, as follows:

Solution	Ingredients
1	1ml of the O/N culture & 4ml of selective LB
2	1ml of the solution 1 & 4ml of the LB
3	1ml of the solution 2 & 4ml of the LB
4	1ml of the solution 3 & 4ml of the LB
5	1ml of the solution 4 & 4ml of the LB