Team:University College London/Protocols/Congo Red Assay

From 2012.igem.org

Curli charcaterisation

Congo red binding assay, Preparation of Congo red supplemented agar:

20uL.ml-1 Congo Red Dye 10ug.ml-1 Coomassie brilliant blue dye G-250 LB Agar (No salt)

Plates subsequently streaked. Uptake of red pigment indicative of amyloid fiber expression.