Team:Technion/19 June 2012
From 2012.igem.org
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Ilya
Inbal
Asaf
Hila
Lior
Noa
Evgeni
Shahar
Rachel
Chemical transformation for competent cells. In this procedure, T7RNAp (p1219 plasmid) and BBa_B0015 (double terminator) were inserted into the cells. The compotent cells were plated on LB+Amp plates. For each transformation, 100 ul of transformed cells were diluted in 900 ul of LB and plated. The cells were incubated at 37 degrees overnight.