Team:Northwestern/Protocols/Overnight

From 2012.igem.org

Overnight Cultures

Procedure

  1. Take 5mL LB and put in a round bottom 5mL tube.
  2. Add 5 μl antibiotic (100x) to the tube.
  3. Take a p200 Tip and lightly touch a colony.
  4. Eject the tip into the culture and put the cap on (not completely).
  5. Grow overnight for 12-16 hours.
  6. 6. Left over overnight cultures (after mini prepping) can be used to make glycerol stocks.

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