"
Team:NTNU Trondheim/Stocks
From 2012.igem.org
This page lists stored cultures and other samples:
Dates are in the format dd.mm.
See also Constructs page.
Contents |
Stored samples
Resuspended DNA
(Dried DNA from distribution kit resuspended in dH2O)
| Name/abbreviation | Date stored | Description | Source | Backbone |
|---|---|---|---|---|
| LR | ? | <partinfo>BBa_C0062</partinfo> | (RS) | <partinfo>pSB1A2</partinfo> |
| Lysis | 22.06 | BBa_K112808 | (RS) | <partinfo>pSB1A2</partinfo> |
| C78 | ? | <partinfo>BBa_C0078</partinfo> | (RS) | <partinfo>pSB2K3</partinfo> |
| C79 | ? | <partinfo>BBa_C0079</partinfo> | (RS) | <partinfo>pSB2K3</partinfo> |
| RBS | 24.06 | <partinfo>BBa_B0034</partinfo> | (RS) | <partinfo>pSB1A2</partinfo> |
| LuxI | ? | <partinfo>BBa_C0061</partinfo> | (RS) | <partinfo>pSB1A2</partinfo> |
| K+RBS | ? | <partinfo>BBa_K081005</partinfo> | (RS) | <partinfo>pSB1A2</partinfo> |
Isolated DNA
(Miniprep, gel extraction and PCR products)
NOT included here: Digestion and ligation mixtures/products
| Name/abbreviation | Date stored | Description | Concentration | Backbone |
|---|---|---|---|---|
| pTet GFP | 26.07 | <partinfo>BBa_I13522</partinfo> | <partinfo>pSB1A2</partinfo> | |
| VGB+RBS+YFP+DTT | 26.07 | |||
| VHB+RBS+YFP+DTT | 26.07 | |||
| EYFP (RBS+ LVA- TERM) | 26.07 | <partinfo>BBa_E0430</partinfo> | pSB1A2 | |
| LuxR+Term | 16.7 | <partinfo>BBa_C0062</partinfo> + <partinfo>BBa_B0015</partinfo> | 37,0 | <partinfo>pSB1AK3</partinfo> |
| LuxI+Term | 16.7 | <partinfo>BBa_C0061</partinfo> + <partinfo>BBa_B0015</partinfo> | 37,6 | <partinfo>pSB1AK3</partinfo> |
| LI (BBa_K092400) | ? | <partinfo>BBa_K092400</partinfo> | <partinfo>pSB1A2</partinfo> | |
| GFP+DTT | 13.07 | <partinfo>BBa_K082003</partinfo>+<partinfo>BBa_B0015</partinfo> | 41,1 | <partinfo>pSB1AK3</partinfo> |
| YFP+DTT | 13.07 | <partinfo>BBa_E0030</partinfo>+<partinfo>BBa_B0015</partinfo> | 55,5 | <partinfo>pSB1AK3</partinfo> |
| RFP+DTT | 13.07 | <partinfo>BBa_E1010</partinfo>+<partinfo>BBa_B0015</partinfo> | 40,9 | <partinfo>pSB1AK3</partinfo> |
| CFP+DTT | 13.07 | <partinfo>BBa_E0020</partinfo>+<partinfo>BBa_B0015</partinfo> | 47,7 | <partinfo>pSB1AK3</partinfo> |
| K+RBS | 13.07 | <partinfo>BBa_K081005</partinfo> | 19,8 | <partinfo>pSB1A2</partinfo> |
| DLD + RBS | 09.07 | <partinfo>BBa_K284003</partinfo> + <partinfo>BBa_B0034</partinfo> | 24,1 | <partinfo>pSB1A2</partinfo> |
| VHB + RBS | 09.07 | <partinfo>BBa_K258005</partinfo> + <partinfo>BBa_B0034</partinfo> | 48,3 | <partinfo>pSB1A2</partinfo> |
| Jen1 + RBS | 09.07 | <partinfo>BBa_K284002</partinfo> + <partinfo>BBa_B0034</partinfo> | 35,3 | <partinfo>pSB1A2</partinfo> |
| VGB + RBS | 09.07 | <partinfo>BBa_K561001</partinfo> + <partinfo>BBa_B0034</partinfo> | 40,0 | <partinfo>pSB1A2</partinfo> |
| pBAD+lysis | 09.07 | 23,6 | <partinfo>pSB1A3</partinfo> | |
| RBS | 06.07 | <partinfo>BBa_B0034</partinfo> | 44,5 | <partinfo>pSB1A2</partinfo> |
| LuxI | 06.07 | <partinfo>BBa_C0061</partinfo> | 38,6 | <partinfo>pSB1A2</partinfo> |
| Colicin | 04.07 | <partinfo>BBa_K150009</partinfo> | 58,6 | <partinfo>pSB1A2</partinfo> |
| VHB | 04.07 | <partinfo>BBa_K258005</partinfo> | 44,9 | <partinfo>pSB1A2</partinfo> |
| Jen1 | 04.07 | <partinfo>BBa_K284002</partinfo> | 102,1 | <partinfo>BBa_J63010</partinfo> |
| DLD | 04.07 | <partinfo>BBa_K284003</partinfo> | 63,8 | <partinfo>BBa_J63010</partinfo> |
| pBAD B1 | 01.07 | <partinfo>BBa_K206000</partinfo> | 24,0 | <partinfo>pSB1A3</partinfo> |
| pBAD B2 | 01.07 | <partinfo>Bba_K206000</partinfo> | 19 | <partinfo>pSB1A3</partinfo> |
| LR | ? | <partinfo>BBa_C0062</partinfo> | 20,3 | <partinfo>pSB1A2</partinfo> |
| VGB promoter | 27.06 | <partinfo>BBa_K561001</partinfo> | 22,9 | <partinfo>pSB1C3</partinfo> |
| C79 | 27.06 | <partinfo>BBa_C0079</partinfo> | 70,8 | <partinfo>pSB2K3</partinfo> |
| C78 | 27.06 | <partinfo>BBa_C0078</partinfo> | 63,4 | <partinfo>pSB2K3</partinfo> |
| RBS | 27.06 | <partinfo>BBa_B0034</partinfo> | 30,2 | <partinfo>pSB1A2</partinfo> |
| RBS | 26.06 | <partinfo>BBa_B0034</partinfo> | 16,8 | <partinfo>pSB1A2</partinfo> |
| DTT Amp | 26.06 | <partinfo>BBa_B0015</partinfo> | 35,8 | <partinfo>pSB1AK3</partinfo> |
| DTT Kan | 26.06 | <partinfo>BBa_B0015</partinfo> | 26,5 | <partinfo>pSB1AK3</partinfo> |
| VGB DNA | 25.06 | <partinfo>BBa_K561001</partinfo> | <partinfo>pSB1C3</partinfo> | |
| luxR+HSL | 25.06 | <partinfo>BBa_R0062</partinfo> | 13,3 | <partinfo>pSB1A2</partinfo> |
| Lysis1 DNA | 24.06 | <partinfo>BBa_K112808</partinfo> | <partinfo>pSB1A2</partinfo> | |
| LuxR+HSL | 24.06 | <partinfo>BBa_R0062</partinfo> | <partinfo>pSB1A2</partinfo> | |
| DNA lysis | 24.06 | <partinfo>BBa_K112808</partinfo> | <partinfo>pSB1A2</partinfo> | |
| Constitutive promoter | 22.06 | <partinfo>BBa_J23119</partinfo> | 27,7 | <partinfo>pSB1A2</partinfo> |
| D2 | 22.6 | <partinfo>BBa_K145150</partinfo> | 23,1 | <partinfo>pSB1A2</partinfo> |
| D1 | 22.06 | <partinfo>BBa_K091146</partinfo> | 19,8 | <partinfo>pSB1A2</partinfo> |
| RFP K | 22.06 | <partinfo>BBa_E1010</partinfo> | <partinfo>pSB2K3</partinfo> | |
| GFP AK | 22.06 | <partinfo>BBa_K082003</partinfo> | <partinfo>pSB1AK3</partinfo> | |
| CFP A | 22.06 | <partinfo>BBa_E0020</partinfo> | <partinfo>pSB1A2</partinfo> | |
| YFP AK | 22.06 | <partinfo>BBa_E0030</partinfo> | <partinfo>pSB1AK3</partinfo> |
Restriction digests and ligation mixtures
(Inserts and backbones, DNA treated with enzymes)
| Name/abbreviation | Date stored | Description | Enzymes | |
|---|---|---|---|---|
| YFP+Term (religation) | 11.07 | YFP+term (religated) | ? | <partinfo>pSB1AK3</partinfo> |
| GFP+Term (religation) | 11.07 | <partinfo>BBa_K082003</partinfo> + <partinfo>BBa_B0015</partinfo> | <partinfo>pSB1AK3</partinfo> | |
| CFP+Term (religation) | 11.07 | <partinfo>BBa_E0020</partinfo> + <partinfo>BBa_B0015</partinfo> | <partinfo>pSB1AK3</partinfo> | |
| RFP +Term (religation) | 11.07 | <partinfo>Bba_E1010</partinfo>+<partinfo>BBa_B0015</partinfo> | <partinfo>pSB1AK3</partinfo> |
Glycerol stocks
(Cell cultures kept at -80 C)
| Name/abbreviation | Date stored | Strain | Plasmid | Position |
|---|---|---|---|---|
| K+RBS+RFP+DTT | ? | DH5a | ? | C1 |
| K+RBS+GFP+DTT | ? | DH5a | ? | C2 |
| RBS (<partinfo>BBa_B0034</partinfo>) | ? | DH5a | ? | C3 |
| pTet mRFP (<partinfo>BBa_I13521</partinfo>) | 27.07 | DH5a | <partinfo>pSB1A2</partinfo>C6 | |
| pTet GFP (<partinfo>BBa_I13522</partinfo>) | 27.07 | DH5a | <partinfo>pSB1A2</partinfo>C5 | |
| EYFP (RBS+ LVA- TERM) (<partinfo>BBa_E0430</partinfo>) | 27.07 | DH5a | <partinfo>pSB1A2</partinfo> | C4 |
| RFP (<partinfo>BBa_E1010</partinfo>) | 18.07 | DH5a | <partinfo>pSB2K3</partinfo> | B6 |
| VGB (<partinfo>BBa_K561001</partinfo>) | 18.07 | DH5a | <partinfo>pSB1C3</partinfo> | B5 |
| LuxR+HSL (<partinfo>BBa_R0062</partinfo>) | 16.07 | DH5a | <partinfo>pSB1A2</partinfo> | A1 |
| Lysis (<partinfo>BBa_K112808</partinfo>) | 16.07 | DH5a | <partinfo>pSB1A2</partinfo> | A3 |
| pBAD (<partinfo>Bba_K206000</partinfo>) | 16.07 | DH5a | <partinfo>pSB1A3</partinfo> | A4 |
| LuxR (<partinfo>BBa_C0062</partinfo>) | 16.07 | DH5a | <partinfo>pSB1A2</partinfo> | A2 |
| GFP (<partinfo>BBa_K082003</partinfo>) | 16.07 | DH5a | <partinfo>pSB1AK3</partinfo> | A5 |
| pBAD+Lysis | 16.07 | DH5a | <partinfo>pSB1A3</partinfo> | A6 |
| DTT (<partinfo>BBa_B0015</partinfo> | 17.07 | DH5a | <partinfo>pSB1AK3</partinfo> | B1 |
| D2 (<partinfo>BBa_K145150</partinfo>) | 17.07 | DH5a | <partinfo>pSB1A2</partinfo> | B4 |
| CFP (<partinfo>BBa_E0020</partinfo>) | 17.07 | DH5a | <partinfo>pSB1A2</partinfo> | B2 |
| YFP (<partinfo>BBa_E0030</partinfo>) | 17.07 | DH5a | <partinfo>pSB1AK3</partinfo> | B3 |
PCR Products
| Name | Target | Primers | Template | Status |
|---|---|---|---|---|
| pllD | pllD promoter | K-12 | Gel OK. Ligated and transformed, but unconfirmed. | |
| Colicin | Colicin operon | ? | Gel good - strong band. |
Agar plates
| Name/abbreviation | Date made | Description | Antibiotic | Plasmid |
|---|---|---|---|---|
| EYFP+RBS+Term | 24.07 | <partinfo>BBa_E0430</partinfo> | Amp | |
| A. vic. GFP | 25.07 | <partinfo>BBa_E0040</partinfo> | Amp | |
| Kolisin | 2.07 | <partinfo>BBa_K150009</partinfo> | Amp | |
| pTet GFP | 24.07 | <partinfo>BBa_I13522</partinfo> | Amp | |
| pTet mRFP | 22.07 | <partinfo>BBa_I13521</partinfo> | Amp | |
| psB1C3 religering | 27.07 | Cm | pSB1C3 | |
| pllD in pSB1C3 | 27.07 | Cm | pSB1C3 | |
| BBa_E1010 | 17.07 | Kan | ||
| VGB | 17.07 | BBa_K561001 | Cm | |
| CFP | 16.07 | Amp | ||
| DTT | 16.07 | BBa_B0015 | Kan | |
| YFP | 16.07 | Amp | ||
| VHB+RBS+YFP+DTT | 24.07 | VHB test construct | Amp | |
| VGB+RBS+YFP+DTT | 24.07 | VGB test construct | Amp | |
| RBS backbone | 03.07 | ? | Amp | |
| GFP + term (DTT) | 11.7 | Amp | ||
| Jen1 | 02.07 | <partinfo>BBa_K284002</partinfo> | Amp | |
| BBa_B0034 | 22.7 | RBS | Amp | |
| K+RBS+GFP+DTT | 18.07 | Amp | ||
| VHB | 02.07 | <partinfo>BBa_K258005</partinfo> | Amp | |
| LuxR | 15.07 | <partinfo>BBa_C0061</partinfo> | Amp | |
| pBAD + lysis | 15.07 | <partinfo>Bba_K206000</partinfo>+<partinfo>BBa_K112808</partinfo> | Amp | |
| Lysis | 15.07 | <partinfo>BBa_K112808</partinfo> | Amp | |
| GFP | 15.07 | ? | Amp | |
| dld | 02.07 | <partinfo>BBa_K284002</partinfo> | Amp | |
| RBS Backbone + VGB | 03.07 | Amp | ||
| YFP + DTT | 11.07 | Amp | ||
| GFP + DTT | 11.07 | Amp | ||
| RFP + DTT | 11.07 | Amp | ||
| K+RBS+YFP+DTT | 17.07 | Amp | ||
| VGB+RBS+GFP+DTT | 17.07 | Amp | ||
| VGB+RBS+RFP+DTT | 17.07 | Amp | ||
| pBAD | 16.07 | Amp | ||
| Jen1+RBS+GFP+DTT | 17.07 | Amp | ||
| LuxR+HSL | 15.07 | Amp | ||
| VHB + RBS | 06.07 | Amp | ||
| VGB+RBS | 06.07 | Amp | ||
| dld+RBS | 06.07 | Amp | ||
| RBS backbone + VGB | 03.07 | Amp | ||
| Jen1+RBS | 06.07 | Amp | ||
| LuxR+DTT | 12.07 | <partinfo>BBa_C0062</partinfo>+<partinfo>BBa_B0015</partinfo> | Amp | |
| LuxI+DTT | 12.07 | Amp | ||
| K+RBS | 11.07 | <partinfo>BBa_K081005</partinfo> | Amp |
Agar plate log:
