From 2012.igem.org

[http://jb.asm.org/content/191/13/4251.abstract The ldhA Gene, Encoding Fermentative l-Lactate Dehydrogenase of Corynebacterium glutamicum, Is under the Control of Positive Feedback Regulation Mediated by LldR

Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif

Promoters of Corynebacterium glutamicum

C. glutamicum lldR crystal structure

RNA polymerase:

Oriented, active Escherichia coli RNA polymerase: an atomic force microscope study

Sequence Specific Interaction between Promoter DNA and Escherichia coli RNA Polymerase: Comparative Thermodynamic Analysis with One Immobilized Partner

Rapid purification of His(6)-tagged Bacillus subtilis core RNA polymerase

Wang et al, 1995: Discontinuous movements of DNA and RNA in RNA polymerase accompany formation of a paused transcription complex

DNA oligo and gene synthesis

http://www.invitrogen.com/site/us/en/home/Products-and-Services/Product-Types/Primers-Oligos-Nucleotides/invitrogen-custom-dna-oligos/Technical-Resources-for-Oligonucleotides/DNA-Oligo-FAQ.html#1

PCR

PCR Principles and Practices

New England Biolabs: PCR Guidelines

http://www.uq.edu.au/vdu/PDU_PCRPrimers.htm

Premier biosoft: PCR primer design guidelines

http://www.cybertory.org/exercises/primerDesign/index.html

http://www.mcdb.lsa.umich.edu/labs/maddock/protocols/PCR/general_pcr_protocol.html

10 tips for designing PCR primers that work

How to make oligonucleotide primers for PCR

Software

http://molbiol-tools.ca/molecular_biology_freeware.htm

quorum sensing

Quorum sensing in bacteria - Annu. Rev. Microbiol. 2001. 55:165–99

N-acyl-L-homoserine lactone signal interception by Escherichia coli.

Other

Syntetisk biologi: Genmodifisert bakterie drep annan bakterie

http://en.wikipedia.org/wiki/DNA_ligase

@@ Line 12: / Line 12: @@
-===Useful links===
+==Useful links==
 http://www.nanodrop.com/Support.aspx?Type=Maintenance&Cat=NanoDrop%201000
@@ Line 27: / Line 27: @@
 [http://www.jbioleng.org/content/5/1/17 Amplified insert assembly: an optimized approach to standard assembly of BioBrickTM genetic circuits]
+[http://www.sbkb.org/tt/  Structural Biology Target Registration Database]
-Lactate genes and promoters:
+[http://dnasu.asu.edu/DNASU/ DNASU plasmid repository]
+[http://www.addgene.org/ Addgene plasmid repository]
+[http://plasmid.med.harvard.edu/PLASMID/ PlasmID database]
+[http://www.chuq.qc.ca/labomoss/english%20pages/protocols.html Moss lab protocols]
+===Lactate genes and promoters:===
 [http://www.biomedcentral.com/1471-2180/10/321#B24 Quinone-dependent D-lactate dehydrogenase Dld (Cg1027) is essential for growth of Corynebacterium glutamicum on D-lactate]
@@ Line 43: / Line 53: @@
 [http://www.rcsb.org/pdb/explore/explore.do?structureId=2di3 C. glutamicum lldR crystal structure]
+RNA polymerase:
+[http://www.ncbi.nlm.nih.gov/pubmed/9916034 Oriented, active Escherichia coli RNA polymerase: an atomic force microscope study]
+[http://pubs.acs.org/doi/abs/10.1021/jp9071197 Sequence Specific Interaction between Promoter DNA and Escherichia coli RNA Polymerase: Comparative Thermodynamic Analysis with One Immobilized Partner]
+[http://www.ncbi.nlm.nih.gov/pubmed/10910724 Rapid purification of His(6)-tagged Bacillus subtilis core RNA polymerase]
+[http://www.sciencedirect.com/science/article/pii/0092867495903879 Wang et al, 1995: Discontinuous movements of DNA and RNA in RNA polymerase accompany formation of a paused transcription complex]
 ==DNA oligo and gene synthesis==

User talk:Jarlemag

From 2012.igem.org

Latest revision as of 19:04, 24 July 2012

Contents

Tasks

Useful links

Lactate genes and promoters:

DNA oligo and gene synthesis

PCR

Software

quorum sensing

Other