User talk:Jarlemag
From 2012.igem.org
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[http://www.sciencedirect.com/science/article/pii/S016816560300155X Promoters of Corynebacterium glutamicum] | [http://www.sciencedirect.com/science/article/pii/S016816560300155X Promoters of Corynebacterium glutamicum] | ||
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+ | [http://www.rcsb.org/pdb/explore/explore.do?structureId=2di3 C. glutamicum lldR crystal structure] | ||
==DNA oligo and gene synthesis== | ==DNA oligo and gene synthesis== |
Revision as of 18:58, 23 July 2012
Contents |
Tasks
- Make cloramphenicol stock. Check.
- Make glycerol stocks of important parts. Discard old plates. Check.
- Perform CFU-assay of lysis induced cells. Check. Redo?
- Combine LuxR-HSL and lysis device.
- Combine pBAD and GFP
- Research lactate production in E. coli
- Write more for the book chapter
- Research QuikChange
Useful links
http://www.nanodrop.com/Support.aspx?Type=Maintenance&Cat=NanoDrop%201000
http://www.troemner.com/pdf/PipetteStandardsHandbook.pdf'
http://www.pipette.com/Support/GuideToPipetting.pdf
https://2008.igem.org/Team:EPF-Lausanne/2-step_PCR
https://2009.igem.org/Team:UNIPV-Pavia/Parts_Characterization#Microplate_reader_experiments
Amplified insert assembly: an optimized approach to standard assembly of BioBrickTM genetic circuits
Lactate genes and promoters:
[http://jb.asm.org/content/191/13/4251.abstract The ldhA Gene, Encoding Fermentative l-Lactate Dehydrogenase of Corynebacterium glutamicum, Is under the Control of Positive Feedback Regulation Mediated by LldR
Promoters of Corynebacterium glutamicum
C. glutamicum lldR crystal structure
DNA oligo and gene synthesis
PCR
New England Biolabs: PCR Guidelines
http://www.uq.edu.au/vdu/PDU_PCRPrimers.htm
Premier biosoft: PCR primer design guidelines
http://www.cybertory.org/exercises/primerDesign/index.html
http://www.mcdb.lsa.umich.edu/labs/maddock/protocols/PCR/general_pcr_protocol.html
10 tips for designing PCR primers that work
How to make oligonucleotide primers for PCR
Software
http://molbiol-tools.ca/molecular_biology_freeware.htm
quorum sensing
Quorum sensing in bacteria - Annu. Rev. Microbiol. 2001. 55:165–99
N-acyl-L-homoserine lactone signal interception by Escherichia coli.
Other
Syntetisk biologi: Genmodifisert bakterie drep annan bakterie
http://en.wikipedia.org/wiki/DNA_ligase