User talk:Jarlemag
From 2012.igem.org
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[http://jb.asm.org/content/191/13/4251.abstract The ldhA Gene, Encoding Fermentative l-Lactate Dehydrogenase of Corynebacterium glutamicum, Is under the Control of Positive Feedback Regulation Mediated by LldR | [http://jb.asm.org/content/191/13/4251.abstract The ldhA Gene, Encoding Fermentative l-Lactate Dehydrogenase of Corynebacterium glutamicum, Is under the Control of Positive Feedback Regulation Mediated by LldR | ||
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+ | [http://mic.sgmjournals.org/content/142/5/1297 Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif] | ||
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+ | [http://www.sciencedirect.com/science/article/pii/S016816560300155X Promoters of Corynebacterium glutamicum] | ||
==DNA oligo and gene synthesis== | ==DNA oligo and gene synthesis== |
Revision as of 19:42, 19 July 2012
Contents |
Tasks
- Make cloramphenicol stock. Check.
- Make glycerol stocks of important parts. Discard old plates. Check.
- Perform CFU-assay of lysis induced cells. Check. Redo?
- Combine LuxR-HSL and lysis device.
- Combine pBAD and GFP
- Research lactate production in E. coli
- Write more for the book chapter
- Research QuikChange
Useful links
http://www.nanodrop.com/Support.aspx?Type=Maintenance&Cat=NanoDrop%201000
http://www.troemner.com/pdf/PipetteStandardsHandbook.pdf'
http://www.pipette.com/Support/GuideToPipetting.pdf
https://2008.igem.org/Team:EPF-Lausanne/2-step_PCR
https://2009.igem.org/Team:UNIPV-Pavia/Parts_Characterization#Microplate_reader_experiments
Amplified insert assembly: an optimized approach to standard assembly of BioBrickTM genetic circuits
Lactate genes and promoters:
[http://jb.asm.org/content/191/13/4251.abstract The ldhA Gene, Encoding Fermentative l-Lactate Dehydrogenase of Corynebacterium glutamicum, Is under the Control of Positive Feedback Regulation Mediated by LldR
Promoters of Corynebacterium glutamicum
DNA oligo and gene synthesis
PCR
New England Biolabs: PCR Guidelines
http://www.uq.edu.au/vdu/PDU_PCRPrimers.htm
Premier biosoft: PCR primer design guidelines
http://www.cybertory.org/exercises/primerDesign/index.html
http://www.mcdb.lsa.umich.edu/labs/maddock/protocols/PCR/general_pcr_protocol.html
10 tips for designing PCR primers that work
How to make oligonucleotide primers for PCR
Software
http://molbiol-tools.ca/molecular_biology_freeware.htm
quorum sensing
Quorum sensing in bacteria - Annu. Rev. Microbiol. 2001. 55:165–99
N-acyl-L-homoserine lactone signal interception by Escherichia coli.
Other
Syntetisk biologi: Genmodifisert bakterie drep annan bakterie
http://en.wikipedia.org/wiki/DNA_ligase