User talk:Jarlemag

From 2012.igem.org

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[http://www.jbioleng.org/content/5/1/17 Amplified insert assembly: an optimized approach to standard assembly of BioBrickTM genetic circuits]
[http://www.jbioleng.org/content/5/1/17 Amplified insert assembly: an optimized approach to standard assembly of BioBrickTM genetic circuits]
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Lactate genes and promoters:
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[http://www.biomedcentral.com/1471-2180/10/321#B24 Quinone-dependent D-lactate dehydrogenase Dld (Cg1027) is essential for growth of Corynebacterium glutamicum on D-lactate]
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[http://aem.asm.org/content/71/10/5920.full Characterization of a Corynebacterium glutamicum Lactate Utilization Operon Induced during Temperature-Triggered Glutamate Production†]
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[http://www.genome.jp/dbget-bin/www_bget?cgb:cg1027 KEGG: cg1027]
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[http://jb.asm.org/content/191/13/4251.abstract The ldhA Gene, Encoding Fermentative l-Lactate Dehydrogenase of Corynebacterium glutamicum, Is under the Control of Positive Feedback Regulation Mediated by LldR
==DNA oligo and gene synthesis==
==DNA oligo and gene synthesis==

Revision as of 19:41, 19 July 2012

Contents

Tasks

  • Make cloramphenicol stock. Check.
  • Make glycerol stocks of important parts. Discard old plates. Check.
  • Perform CFU-assay of lysis induced cells. Check. Redo?
  • Combine LuxR-HSL and lysis device.
  • Combine pBAD and GFP
  • Research lactate production in E. coli
  • Write more for the book chapter
  • Research QuikChange


Useful links

http://www.nanodrop.com/Support.aspx?Type=Maintenance&Cat=NanoDrop%201000

http://www.troemner.com/pdf/PipetteStandardsHandbook.pdf'

http://www.pipette.com/Support/GuideToPipetting.pdf


https://2008.igem.org/Team:EPF-Lausanne/2-step_PCR

https://2009.igem.org/Team:UNIPV-Pavia/Parts_Characterization#Microplate_reader_experiments

Amplified insert assembly: an optimized approach to standard assembly of BioBrickTM genetic circuits


Lactate genes and promoters:

Quinone-dependent D-lactate dehydrogenase Dld (Cg1027) is essential for growth of Corynebacterium glutamicum on D-lactate

Characterization of a Corynebacterium glutamicum Lactate Utilization Operon Induced during Temperature-Triggered Glutamate Production†

KEGG: cg1027

[http://jb.asm.org/content/191/13/4251.abstract The ldhA Gene, Encoding Fermentative l-Lactate Dehydrogenase of Corynebacterium glutamicum, Is under the Control of Positive Feedback Regulation Mediated by LldR

DNA oligo and gene synthesis

http://www.invitrogen.com/site/us/en/home/Products-and-Services/Product-Types/Primers-Oligos-Nucleotides/invitrogen-custom-dna-oligos/Technical-Resources-for-Oligonucleotides/DNA-Oligo-FAQ.html#1

PCR

PCR Principles and Practices

New England Biolabs: PCR Guidelines

http://www.uq.edu.au/vdu/PDU_PCRPrimers.htm

Premier biosoft: PCR primer design guidelines

http://www.cybertory.org/exercises/primerDesign/index.html

http://www.mcdb.lsa.umich.edu/labs/maddock/protocols/PCR/general_pcr_protocol.html

10 tips for designing PCR primers that work

How to make oligonucleotide primers for PCR


Software

http://molbiol-tools.ca/molecular_biology_freeware.htm


quorum sensing

Quorum sensing in bacteria - Annu. Rev. Microbiol. 2001. 55:165–99

N-acyl-L-homoserine lactone signal interception by Escherichia coli.


Other

Syntetisk biologi: Genmodifisert bakterie drep annan bakterie

http://en.wikipedia.org/wiki/DNA_ligase

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