User:Jn323/25 June 2012

From 2012.igem.org

Revision as of 00:00, 4 October 2012 by Ajl58 (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Cell Transformation

  • Thaw Origami cells on ice
  • Aliquot 50uL of competent cells into two pre-chilled 15mL culture tubes
  • Add DNA (1-5ng in 1-5uL) to one of the culture tubes (other culture tube being a control)
  • Swirl gently with pipette tip
  • Chill tubes on ice for 30 minutes
  • Heat pulse tubes at 42°C for 30-40 seconds
  • Chill tubes again on ice for 2 minutes
  • Add 500uL of LB media into each tube
  • Culture cells in tubes in a 37° incubator while shaking
  • Plate cultures on Amp/Kan plates and incubate at 37°C
Results