Team:WashU/Week6
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==YLC Color Constructs== | ==YLC Color Constructs== | ||
- | Since the color constructs are proving difficult, and since we will not have the primers until Friday, we are rehydrating and transforming 4 others constructs to have the colors ready for the YLC project demonstration in one week. The following 4 parts were used to transform cells that will incubate at 37°C overnight:<br> | + | Since the color constructs are proving difficult, and since we will not have the primers until Friday, we are rehydrating and transforming 4 others constructs to have the colors ready for the YLC project demonstration in one week. The following 4 parts were used to transform cells that will incubate at 37°C overnight:<br><br> |
RFP: I13521<br> | RFP: I13521<br> | ||
GFP: I13522<br> | GFP: I13522<br> | ||
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+ | ==YLC Color Constructs== | ||
+ | The plates all have a good amount of colonies on them. Here are pictures of them in light and under UV in darkness:<br>PICTURES!<br> | ||
+ | The RFP and GFP are glowing well after about 14 hours of incubation. They are now sitting and room temperature while the CFP and YFP plates are placed back in the incubator to further develop. | ||
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+ | The GFP and RFP plates both had colonies picked from them to prepare in Amp liquid culture. These will be used to create sample plates for the YLC and start preparing solutions with which the campers at the YLC will BioPaint. | ||
+ | ==Saffron Constructs Preparation== | ||
+ | Today, the Kan-plasmid which we are using as the construct vector and the CS42s construct to produce Saffron from Zeaxanthin were both digested with XbaI and SpeI. These digests were then run on a gel to prepare for gel purification. The gel from yesterday was not a clear gel result so this gel was a 3.5% gel to try to further see separation of bands.<br> | ||
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Revision as of 15:55, 3 July 2012