Team:WashU/Week2
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Five biobricks used for transformations this week: | Five biobricks used for transformations this week: | ||
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6. YFP: <a href="http://partsregistry.org/Part:BBa_I13003:Design">BBa_I13003</a> | 6. YFP: <a href="http://partsregistry.org/Part:BBa_I13003:Design">BBa_I13003</a> | ||
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4. Old eCFP: <a href="http://www.google.com/url?q=http%3A%2F%2Fpartsregistry.org%2Fwiki%2Findex.php%3Ftitle%3DPart%3ABBa_E0420&sa=D&sntz=1&usg=AFQjCNGqh4nrV86rOPlesY61Pf7iLz8FNA">BBa_E0420</a> | 4. Old eCFP: <a href="http://www.google.com/url?q=http%3A%2F%2Fpartsregistry.org%2Fwiki%2Findex.php%3Ftitle%3DPart%3ABBa_E0420&sa=D&sntz=1&usg=AFQjCNGqh4nrV86rOPlesY61Pf7iLz8FNA">BBa_E0420</a> | ||
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Afterwards, we grew the cells up on plates with ampicillin, picked colonies and prepared liquid cultures. Then, we prepared minipreps for all of the transformed cells and ran a gel. The results are shown below. | Afterwards, we grew the cells up on plates with ampicillin, picked colonies and prepared liquid cultures. Then, we prepared minipreps for all of the transformed cells and ran a gel. The results are shown below. | ||
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[[file:1,2,3,4,5,6,7,8 gel.jpg|thumb|x350px|left|Agarose gel of DNA to make fluorescent proteins ("the gel master's" gel)]] | [[file:1,2,3,4,5,6,7,8 gel.jpg|thumb|x350px|left|Agarose gel of DNA to make fluorescent proteins ("the gel master's" gel)]] | ||
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<font size = "4"><u>Website work:</u></font> | <font size = "4"><u>Website work:</u></font> | ||
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Each team member completed his biography, after which the webmaster uploaded them to the website. Work was also done on other parts of the website, including the header, safety page, sponsor section, and media page. The team plans to post pictures on the website detailing all of the misadventures of the iGEM 2012 team, and may even delve into film, should time permit. Finally, plans are in the air for a potential new logo and new banner for the website. | Each team member completed his biography, after which the webmaster uploaded them to the website. Work was also done on other parts of the website, including the header, safety page, sponsor section, and media page. The team plans to post pictures on the website detailing all of the misadventures of the iGEM 2012 team, and may even delve into film, should time permit. Finally, plans are in the air for a potential new logo and new banner for the website. | ||
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<font size = "4"><u>Saffron in a Kan: Synechocystis:</u></font> | <font size = "4"><u>Saffron in a Kan: Synechocystis:</u></font> | ||
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In the end, these colonies did not produce the results that we wanted. A second attempt will be made next week. | In the end, these colonies did not produce the results that we wanted. A second attempt will be made next week. | ||
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<font size ="5"><a href="https://2012.igem.org/Team:WashU/WeeklyLog">Back to Weekly Log</a></font> | <font size ="5"><a href="https://2012.igem.org/Team:WashU/WeeklyLog">Back to Weekly Log</a></font> | ||
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Latest revision as of 15:34, 9 August 2012