Monday, August 6
Colony PCR of U construct using four new colonies and the same positive Z control has once again resulted in no successful colonies. However, the PCR has reaffirmed that the control works, which is a good sign.
We also did a PCR of the color constructs in order to form our BioPaint set. [PICTURE BELOW]
Since our T plasmid and Z construct ligations have not been going well, we started over by preparing new cultures of Z and T, running them on a gel, and then gel purifying the results. [SEE PICTURE]
We began 5 ml cultures for the good Z construct with TOPO and for one of the U ligation colonies. We will use the Z culture for a glycerol stock, and digest the U culture to see if our ligations actually succeded.
Tuesday, August 7
Wednesday, August 8
Thursday, August 9
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