Team:WashU/Protocols/LBPlates
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#Autoclave mixture for 20 minutes on liquid cycle. | #Autoclave mixture for 20 minutes on liquid cycle. | ||
#Allow mixture to cool to around 55 degrees Celsius. | #Allow mixture to cool to around 55 degrees Celsius. | ||
- | #(optional). add appropriate amount of antibiotic. Final concentrations are given. Ampicillin-100ug/ml, Kanamycin-50ug/ml, Tetracycline-15ug/ml, Chloramphenicol- | + | #(optional). add appropriate amount of antibiotic. Final concentrations are given. Ampicillin-100ug/ml, Kanamycin-50ug/ml, Tetracycline-15ug/ml, Chloramphenicol-50ug/ml |
#Pour 25 ml of agar mixture into plates, and allow to solidify. | #Pour 25 ml of agar mixture into plates, and allow to solidify. | ||
#Use, or store in refrigerator. | #Use, or store in refrigerator. |
Revision as of 17:24, 2 July 2012