Team:WashU/Protocols/GlycerolStock

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General Glycerol Stock Protocol


1) Add .5 mL 50% glycerol solution in water and .5 mL cell culture 6-18 hours old (cells preferably still in exponential growth phase) to a 1.5 mL Eppendorf tube.
2) If possible, flash freeze in dry ice bathed in 70% ethanol.
3) Store at -80°C.

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