Team:Uppsala University/Datapage
From 2012.igem.org
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Small RNA ScaffoldBBa_K864440 - J23101-Spot42:This is the native spot42 small RNA constitutively expressed by BBa_J23101 and is useful as a template for generating a randomized library of small RNAs for downregulation of any gene of interest. Reporter SystemBBa_K864444 - RFP-linker-SYFP:This part can be used to insert any 5’UTR of a gene of interest to screen for downregulation of this gene by artificial small RNAs. This reporter reporter system comes with a fluorescent marker that is downregulated by any small RNA targeting the 5'UTR of the gene of interest. New low-copy backboneBBa_K864001 - pSB4C15:This is a new BioBrick standard vector with low copy pSC101 replication origin, especially usable for Lambda Red recombineering in E coli. Blue ChromoproteinBBa_K864401 - aeBlue:aeBlue is a blue chromoprotein extracted from the basal disk of a beadlet anemone Actinia equina. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue has significant sequence homologies with proteins in the GFP family. | |||
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PromotertestBBa_K864401 - T5-lac promoter:We characterized this promoter in different strains as well as in one of our new lacIq backbones. BackbonesBBa_K864401 - aeBlue:eforRedBBa_K864401 - aeBlue: | |||
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catSacBBBa_K864401 - aeBlue:IqBBBBa_K864401 - aeBlue:SP8BBBBa_K864401 - aeBlue: |