Team:Uppsala University/Datapage

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Team Uppsala University – iGEM 2012


System Overview

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System Overview

Data for Favorite Parts

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Small RNA Scaffold

BBa_K864440 - J23101-Spot42: This is the native spot42 small RNA constitutively expressed by BBa_J23101 and is useful as a template for generating a randomized library of small RNAs for downregulation of any gene of interest.

Reporter System

BBa_K864444 - RFP-linker-SYFP: This part can be used to insert any 5’ÚTR of a gene of interest to screen for downregulation of this gene by artificial small RNAs. This reporter reporter system comes with a fluorescent marker that is downregulated by any small RNA targeting the 5'UTR of the gene of interest.

New low-copy backbone

BBa_K864001 - pSB4C15: This is a new BioBrick standard vector with low copy pSC101 replication origin, especially usable for Lambda Red recombineering in E coli.

Blue Chromoprotein

BBa_K864401 - aeBlue:

Data for Pre-existing Parts

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Promotortest

BBa_K864401 - aeBlue:

Backbones

BBa_K864401 - aeBlue:

eforRed

BBa_K864401 - aeBlue:

Other Characterized Parts

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catSacB

BBa_K864401 - aeBlue:

IqBB

BBa_K864401 - aeBlue:

SP8BB

BBa_K864401 - aeBlue:



Sponsors






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