Team:Uppsala University/Datapage

From 2012.igem.org

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(Undo revision 234158 by Erlu2699 (talk))
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<h3><b>Small RNA Scaffold</b></h3>
<h3><b>Small RNA Scaffold</b></h3>
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864440" style="margin-left:10px">BBa_K864440</a> - <b>J23101-Spot42</b>:
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864440" style="margin-left:10px">BBa_K864440</a> - <b>J23101-Spot42</b>:
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<p>This is the native spot42 small RNA constitutively expressed by BBa_J23101 and is useful as a template for generating a randomized library of small RNAs for downregulation of any gene of interest.</p>
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<h3><b>Reporter System</b></h3>
<h3><b>Reporter System</b></h3>
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864444" style="margin-left:10px">BBa_K864444</a> - <b>RFP-linker-SYFP</b>:
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864444" style="margin-left:10px">BBa_K864444</a> - <b>RFP-linker-SYFP</b>:
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<p>This part can be used to insert any 5’UTR of a gene of interest to screen for downregulation of this gene by artificial small RNAs. This reporter reporter system comes with a fluorescent marker that is downregulated by any small RNA targeting the 5'UTR of the gene of interest.</p>
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<h3><b>New low-copy backbone</b></h3>
<h3><b>New low-copy backbone</b></h3>
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864001" style="margin-left:10px">BBa_K864001</a> - <b>pSB4C15</b>:
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864001" style="margin-left:10px">BBa_K864001</a> - <b>pSB4C15</b>:
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<p>This is a new BioBrick standard vector with low copy pSC101 replication origin, especially usable for Lambda Red recombineering in E coli.</p>
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Revision as of 02:59, 27 September 2012

Team Uppsala University – iGEM 2012


System Overview

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System Overview

Data for Favorite Parts

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Small RNA Scaffold

BBa_K864440 - J23101-Spot42:

This is the native spot42 small RNA constitutively expressed by BBa_J23101 and is useful as a template for generating a randomized library of small RNAs for downregulation of any gene of interest.



Reporter System

BBa_K864444 - RFP-linker-SYFP:

This part can be used to insert any 5’UTR of a gene of interest to screen for downregulation of this gene by artificial small RNAs. This reporter reporter system comes with a fluorescent marker that is downregulated by any small RNA targeting the 5'UTR of the gene of interest.



New low-copy backbone

BBa_K864001 - pSB4C15:

This is a new BioBrick standard vector with low copy pSC101 replication origin, especially usable for Lambda Red recombineering in E coli.



Blue Chromoprotein

BBa_K864401 - aeBlue:

Data for Pre-existing Parts

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Promotortest

BBa_K864401 - aeBlue:

Backbones

BBa_K864401 - aeBlue:

eforRed

BBa_K864401 - aeBlue:

Other Characterized Parts

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catSacB

BBa_K864401 - aeBlue:

IqBB

BBa_K864401 - aeBlue:

SP8BB

BBa_K864401 - aeBlue:



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