Team:Uppsala University/Backbones

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(Difference between revisions)

Revision as of 18:59, 10 September 2012

Team Uppsala University – iGEM 2012

Standard low-copy backbones

Registry ID	Name	Ori	Resistance	Insert	Size	Status
	pSB4A15	pSC101	Amp	pUCori-red		Finished
	pSB4C15	pSC101	Cm	pUCori-red		Finished
	pSB4K15	pSC101	Kan	pUCori-red		Planning
	pSB4S15	pSC101	Spec	pUCori-red		Finished
	pSB4T15	pSC101	Tet	pUCori-red		Finished

LaqIq backbones

For expression of toxic genes, or simply genes where you want to be able to tune the expression level, we constructed a series of lacIq bacbones. Including the lacIq casette on the plasmid ensures that the copy number of the repression always follows that of your inserted genes, providing guranteed strong repression without inducing unneccessary metabolic load.

Registry ID	Name	Ori	Resistance	Insert	Size	Status
	pSB4A15Iq	pSC101	Amp	pUCori-redq		Planning
	pSB4C15Iq	pSC101	Cm	pUCori-redq		Planning
	pSB4K15Iq	pSC101	Kan	pUCori-redq		Planning
	pSB4S15Iq	pSC101	Spec	pUCori-redq		Planning
	pSB4T15Iq	pSC101	Tet	pUCori-redq		Planning

Chromosomal intergration backbones

We have made special low copy backbones for recombination onto the bacterial chromosome. The antibiotic resistance casette is flanked by Flp recombinase recognition target sites (FRT sites), meaning that the casette can be flipped out of the chromosome after integration and selection. Since low copy plasmids still have a higher copy number than one, it can be also useful to to repress toxic genes during cloning work with the lacIq system.

Registry ID	Name	Ori	Resistance	Insert	Size	Status
	pSB4A15Flp	pSC101	Amp	pUCori-red		Finished
	pSB4C15Flp	pSC101	Cm	pUCori-red		Finished
	pSB4K15Flp	pSC101	Kan	pUCori-red		Finished
	pSB4S15Flp	pSC101	Spec	pUCori-red		Finished
	pSB4T15Flp	pSC101	Tet	pUCori-red		Finished
	pSB4A15FlpIq	pSC101	Amp	pUCori-redq		Planning
	pSB4C15FlpIq	pSC101	Cm	pUCori-redq		Planning
	pSB4K15FlpIq	pSC101	Kan	pUCori-redq		Planning
	pSB4S15FlpIq	pSC101	Spec	pUCori-redq		Planning
	pSB4T15FlpIq	pSC101	Tet	pUCori-redq		Planning

@@ Line 23: / Line 23: @@
 <tr>
-<td class="subtext"><h2>Standard backbones</h2></td>
+<td class="subtext"><h2>Standard low-copy backbones</h2></td>
 <td valign="bottom"><a id="top" href="#top">Back to top</a></td></tr>
 </table>
@@ Line 35: / Line 35: @@
 <table>
 <tr>
-<td width="100"><b>Registry ID</b></td><td width="100"><b>Name</b></td> <td width="100"><b>Ori<b> </td> <td width="100"><b>Resistance</b></td> <td width="100"><b>Insert</b></td> <td width="100"><b>Status</b></td>
+<td width="100"><b>Registry ID</b></td><td width="100"><b>Name</b></td> <td width="70"><b>Ori<b> </td> <td width="80"><b>Resistance</b></td> <td width="100"><b>Insert</b><td width="70"><b>Size</b></td></td> <td width="100"><b>Status</b></td>
 </tr><tr>
-<td></td><td>pSB4A15</td> <td>pSC101</td><td>Amp</td><td>pUC-red</td><td>Finished</td>
+<td></td><td>pSB4A15</td> <td>pSC101</td><td>Amp</td><td>pUCori-red</td><td></td><td>Finished</td>
 </tr><tr>
-<td></td><td>pSB4C15</td> <td>pSC101</td><td>Cm</td><td>pUC-red</td><td>Finished</td>
+<td></td><td>pSB4C15</td> <td>pSC101</td><td>Cm</td><td>pUCori-red</td><td></td><td>Finished</td>
 </tr><tr>
-<td></td><td>pSB4K15</td> <td>pSC101</td><td>Kan</td><td>pUC-red</td><td>Planning</td>
+<td></td><td>pSB4K15</td> <td>pSC101</td><td>Kan</td><td>pUCori-red</td><td></td><td>Planning</td>
 </tr><tr>
-<td></td><td>pSB4S15</td> <td>pSC101</td><td>Spec</td><td>pUC-red</td><td>Finished</td>
+<td></td><td>pSB4S15</td> <td>pSC101</td><td>Spec</td><td>pUCori-red</td><td></td><td>Finished</td>
 </tr><tr>
-<td></td><td>pSB4T15</td> <td>pSC101</td><td>Tet</td><td>pUC-red</td><td>Finished</td>
+<td></td><td>pSB4T15</td> <td>pSC101</td><td>Tet</td><td>pUCori-red</td><td></td><td>Finished</td>
 </tr>
 </table>
@@ Line 74: / Line 74: @@
 <table>
 <tr>
-<td width="100"><b>Registry ID</b></td><td width="100"><b>Name</b></td> <td width="100"><b>Ori<b> </td> <td width="100"><b>Resistance</b></td> <td width="100"><b>Insert</b></td> <td width="100"><b>Status</b></td>
+<td width="100"><b>Registry ID</b></td><td width="100"><b>Name</b></td> <td width="70"><b>Ori<b> </td> <td width="80"><b>Resistance</b></td> <td width="100"><b>Insert</b><td width="70"><b>Size</b></td></td> <td width="100"><b>Status</b></td>
 </tr><tr>
-<td></td><td>pSB4A15Iq</td> <td>pSC101</td><td>Amp</td><td>pUC-redq</td><td>Planning</td>
+<td></td><td>pSB4A15Iq</td> <td>pSC101</td><td>Amp</td><td>pUCori-redq</td><td></td><td>Planning</td>
 </tr><tr>
-<td></td><td>pSB4C15Iq</td> <td>pSC101</td><td>Cm</td><td>pUC-redq</td><td>Planning</td>
+<td></td><td>pSB4C15Iq</td> <td>pSC101</td><td>Cm</td><td>pUCori-redq</td><td></td><td>Planning</td>
 </tr><tr>
-<td></td><td>pSB4K15Iq</td> <td>pSC101</td><td>Kan</td><td>pUC-redq</td><td>Planning</td>
+<td></td><td>pSB4K15Iq</td> <td>pSC101</td><td>Kan</td><td>pUCori-redq</td><td></td><td>Planning</td>
 </tr><tr>
-<td></td><td>pSB4S15Iq</td> <td>pSC101</td><td>Spec</td><td>pUC-redq</td><td>Planning</td>
+<td></td><td>pSB4S15Iq</td> <td>pSC101</td><td>Spec</td><td>pUCori-redq</td><td></td><td>Planning</td>
 </tr><tr>
-<td></td><td>pSB4T15Iq</td> <td>pSC101</td><td>Tet</td><td>pUC-redq</td><td>Planning</td>
+<td></td><td>pSB4T15Iq</td> <td>pSC101</td><td>Tet</td><td>pUCori-redq</td><td></td><td>Planning</td>
 </tr>
 </table>
@@ Line 114: / Line 114: @@
 <table>
 <tr>
-<td width="100"><b>Registry ID</b></td><td width="100"><b>Name</b></td> <td width="100"><b>Ori<b> </td> <td width="100"><b>Resistance</b></td> <td width="100"><b>Insert</b></td> <td width="100"><b>Status</b></td>
+<td width="100"><b>Registry ID</b></td><td width="100"><b>Name</b></td> <td width="70"><b>Ori<b> </td> <td width="80"><b>Resistance</b></td> <td width="100"><b>Insert</b><td width="70"><b>Size</b></td></td> <td width="100"><b>Status</b></td>
 </tr><tr>
-<td></td><td>pSB4A15Flp</td> <td>pSC101</td><td>Amp</td><td>pUC-red</td><td>Finished</td>
+<td></td><td>pSB4A15Flp</td> <td>pSC101</td><td>Amp</td><td>pUCori-red</td><td></td><td>Finished</td>
 </tr><tr>
-<td></td><td>pSB4C15Flp</td> <td>pSC101</td><td>Cm</td><td>pUC-red</td><td>Finished</td>
+<td></td><td>pSB4C15Flp</td> <td>pSC101</td><td>Cm</td><td>pUCori-red</td><td></td><td>Finished</td>
 </tr><tr>
-<td></td><td>pSB4K15Flp</td> <td>pSC101</td><td>Kan</td><td>pUC-red</td><td>Finished</td>
+<td></td><td>pSB4K15Flp</td> <td>pSC101</td><td>Kan</td><td>pUCori-red</td><td></td><td>Finished</td>
 </tr><tr>
-<td></td><td>pSB4S15Flp</td> <td>pSC101</td><td>Spec</td><td>pUC-red</td><td>Finished</td>
+<td></td><td>pSB4S15Flp</td> <td>pSC101</td><td>Spec</td><td>pUCori-red</td><td></td><td>Finished</td>
 </tr><tr>
-<td></td><td>pSB4T15Flp</td> <td>pSC101</td><td>Tet</td><td>pUC-red</td><td>Finished</td>
+<td></td><td>pSB4T15Flp</td> <td>pSC101</td><td>Tet</td><td>pUCori-red</td><td></td><td>Finished</td>
 </tr><tr>
-<td></td><td>pSB4A15FlpIq</td> <td>pSC101</td><td>Amp</td><td>pUC-redq</td><td>Planning</td>
+<td></td><td>pSB4A15FlpIq</td> <td>pSC101</td><td>Amp</td><td>pUCori-redq</td><td></td><td>Planning</td>
 </tr><tr>
-<td></td><td>pSB4C15FlpIq</td> <td>pSC101</td><td>Cm</td><td>pUC-redq</td><td>Planning</td>
+<td></td><td>pSB4C15FlpIq</td> <td>pSC101</td><td>Cm</td><td>pUCori-redq</td><td></td><td>Planning</td>
 </tr><tr>
-<td></td><td>pSB4K15FlpIq</td> <td>pSC101</td><td>Kan</td><td>pUC-redq</td><td>Planning</td>
+<td></td><td>pSB4K15FlpIq</td> <td>pSC101</td><td>Kan</td><td>pUCori-redq</td><td></td><td>Planning</td>
 </tr><tr>
-<td></td><td>pSB4S15FlpIq</td> <td>pSC101</td><td>Spec</td><td>pUC-redq</td><td>Planning</td>
+<td></td><td>pSB4S15FlpIq</td> <td>pSC101</td><td>Spec</td><td>pUCori-redq</td><td></td><td>Planning</td>
 </tr><tr>
-<td></td><td>pSB4T15FlpIq</td> <td>pSC101</td><td>Tet</td><td>pUC-redq</td><td>Planning</td>
+<td></td><td>pSB4T15FlpIq</td> <td>pSC101</td><td>Tet</td><td>pUCori-redq</td><td></td><td>Planning</td>
 </tr>
 </table>