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Team:University College London/Protocols/Antibiotics
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'''Protocol 1: Ampicillin''' | '''Protocol 1: Ampicillin''' | ||
| - | + | 1.Find the jar of Ampicillin powder in the -20 fridge - there are two - make sure you use the right one for this protocol | |
2.Weigh out 100mg Ampicillin - be as accurate as possible | 2.Weigh out 100mg Ampicillin - be as accurate as possible | ||
Latest revision as of 15:47, 16 July 2012
Making Antibiotics
Protocol 1: Ampicillin
1.Find the jar of Ampicillin powder in the -20 fridge - there are two - make sure you use the right one for this protocol
2.Weigh out 100mg Ampicillin - be as accurate as possible
3.Remove the plastic tray on which you weighed the Ampicillin and put a Falcon tube on the scales
4.Zero the scales
5.Add the powder to the Ampicillin - making sure it comes to the right amount.
6.Concentration is important
7.Remove the Falcon tube and add 500ul of autoclaved water, and 500ul of 100% Ethanol. 8.Put lid on and use syringe to pipette up and down
9.It should dissolve relatively easily
10.Then, using a syringe, suck up the Ampicillin solutution and fit a 200nm filter to the end of the syringe
11.Into another falcon, push the solution through the filter - do this SLOWLY as the filter is easily broken
12.Pipette 1 ml into a tube
13.Obviously if you are making up 5 eppendorfs - you need to do five time the amount described above
