Team:UT Dallas/Project1

From 2012.igem.org

(Difference between revisions)
 
(19 intermediate revisions not shown)
Line 7: Line 7:
<!-- Internet Explorer PNG fix - END /-->
<!-- Internet Explorer PNG fix - END /-->
 +
<link href='http://fonts.googleapis.com/css?family=Scada' rel='stylesheet' type='text/css'>
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jqueryui/1.8.2/jquery-ui.min.js"></script>
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jqueryui/1.8.2/jquery-ui.min.js"></script>
<script type='text/javascript' src='https://2012.igem.org/Team:UT_Dallas/script.js?action=raw&ctype=text/javascript'></script>
<script type='text/javascript' src='https://2012.igem.org/Team:UT_Dallas/script.js?action=raw&ctype=text/javascript'></script>
-
<link href="https://2012.igem.org/Team:UT_Dallas/test/page.css?action=raw&ctype=text/css&purtryjryjytu=eryw" rel="stylesheet">
+
<script type="text/javascript">
-
<script language="javascript">
+
var prev_fname="";
 +
function show_project_info(fname)
 +
{
 +
if(prev_fname!=fname)
 +
{
 +
        $('#st_right').fadeOut("slow", function (){
 +
$.get("https://2012.igem.org/Team:UT_Dallas/"+fname+"?action=raw&ctype=text/x-wiki", function(data) {
 +
$('#st_right').html(data);
 +
$('#st_right').fadeIn("slow");
 +
});
 +
});
 +
prev_fname=fname;
 +
}
 +
}
 +
 
$(document).ready(function(){  colorize_menu('project');});
$(document).ready(function(){  colorize_menu('project');});
</script>
</script>
 +
<link href="https://2012.igem.org/Team:UT_Dallas/test/page.css?action=raw&ctype=text/css" rel="stylesheet">
</html>
</html>
<div id='top_box'>
<div id='top_box'>
Line 18: Line 34:
</div>
</div>
<div id='middle_box'>
<div id='middle_box'>
-
<div id='st_1'><h2 class='title'>Project: Toggle Switch</h2>
+
<div id='st_1'><center><h2 class='title' style="margin-bottom: 18px;margin-top: 7px;">Single Population Toggle Switch</h2></center>
-
For this part of the project, our team created a working toggle switch mechanism with two different colonies of bacteria. Basically, one colony promotes the other colony and vice-versa. I have no idea what else to add here, so I'm just typing stuff.
+
</div>
 +
<div id='st_2'>
 +
  <table class="t1" cellspacing="0" cellpadding="0">
 +
  <tr>
 +
<td id='st_left' valign='top'>
 +
<ul style='list-style: none outside none;'>
 +
  <li class='st_list' onclick="show_project_info('toggle_switch')">One Population</li>
 +
  <li class='st_list' onclick="show_project_info('toggle_switch_design')">Design</li>
 +
  <li class='st_list' onclick="show_project_info('toggle_switch_results')">Data/Results</li>
 +
  <li class='st_list' onclick="show_project_info('toggle_switch_biobricks')">Biobricks</li>
 +
  <li class='st_list' onclick="show_project_info('toggle_switch_future_dir')">Future Direction</li>
 +
</ul>
 +
 
 +
</td>
 +
<td id='st_right' valign='top'><h2 class='title' style='font-size: 120%;'>Overview</h2>Our project is centered around creating a working toggle switch that flips between two different states when presented with certain chemicals. We started with a simple switch that utilizes two inhibitor proteins, LacI and TetR, which bind to sites on the pLac and pTet promoters, respectively. When bound to these promoters transcription is not able to proceed; any genes downstream of the promoter are effectively turned off. However, certain chemicals (IPTG in the case of LacI) will prevent these inhibitor proteins from binding to their respective promoters, allowing transcription of genes to continue constituively. Our design places a fluorescent and inhibitor gene downstream of one of these promoters, as shown in the design section. If these parts work as intended, then side 1, when running, should turn off side 2, and vice versa. By adding the chemicals IPTG or ATc, we can turn off the inhibitor proteins of one side, allowing the other side to become dominant.</td>
 +
</tr>
 +
</table>
</div>
</div>
</div>
</div>
</html>
</html>

Latest revision as of 02:14, 4 October 2012

Single Population Toggle Switch

  • One Population
  • Design
  • Data/Results
  • Biobricks
  • Future Direction

Overview

Our project is centered around creating a working toggle switch that flips between two different states when presented with certain chemicals. We started with a simple switch that utilizes two inhibitor proteins, LacI and TetR, which bind to sites on the pLac and pTet promoters, respectively. When bound to these promoters transcription is not able to proceed; any genes downstream of the promoter are effectively turned off. However, certain chemicals (IPTG in the case of LacI) will prevent these inhibitor proteins from binding to their respective promoters, allowing transcription of genes to continue constituively. Our design places a fluorescent and inhibitor gene downstream of one of these promoters, as shown in the design section. If these parts work as intended, then side 1, when running, should turn off side 2, and vice versa. By adding the chemicals IPTG or ATc, we can turn off the inhibitor proteins of one side, allowing the other side to become dominant.
Retrieved from "http://2012.igem.org/Team:UT_Dallas/Project1"