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Team:UC Chile/Cyano/Notepad/week22
From 2012.igem.org
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<!-- Monday: Carla is back in the lab. And Pollak didn't find a better way to greet her than to smash her watch against the floor. So now he owes her a new watch. | <!-- Monday: Carla is back in the lab. And Pollak didn't find a better way to greet her than to smash her watch against the floor. So now he owes her a new watch. | ||
| - | Also, on the Arduino road... we realize that the sensor we got last week was not the one we asked for u.u Therefore, we have to wait another two weeks to try our arduino light sensing system. Truth to be told, we have a really tiny sensor (milimeters in length)and we really don't know how to wire it because it doesn't have any visible terminals :( Carla is going to Olimex's technical support on Friday. | + | Also, on the Arduino road... we realize that the sensor we got last week was not the one we asked for u.u Therefore, if we ask for the correct sensor we'll have to wait another two weeks to try our arduino light sensing system. Truth to be told, we have a really tiny sensor (milimeters in length)and we really don't know how to wire it because it doesn't have any visible terminals :( Carla is going to Olimex's technical support on Friday. |
Oh... and the most important thing: THERE ARE LIKE A MILLION COLONIES IN THE POSITIVE CONTROL FOR GIBSON :D (psB4K5 + sfGFP) So the problem seemed to be related with the e. clonis we were using. Now with the TOP10 cells we have our faith back :) And it's time to celebrate with good beer of course. | Oh... and the most important thing: THERE ARE LIKE A MILLION COLONIES IN THE POSITIVE CONTROL FOR GIBSON :D (psB4K5 + sfGFP) So the problem seemed to be related with the e. clonis we were using. Now with the TOP10 cells we have our faith back :) And it's time to celebrate with good beer of course. | ||
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We received and interesting e-mail today. Javiera López, a doctoreate student at Bioengineering department realized the concentrations of MgCl2 and DTT in the isothermal buffer for Gibson were exchanged. We are helping her to implement the Gibson technique in her lab, but till now she has proven to be more of help than us! We'll prepare a new buffer ASAP. | We received and interesting e-mail today. Javiera López, a doctoreate student at Bioengineering department realized the concentrations of MgCl2 and DTT in the isothermal buffer for Gibson were exchanged. We are helping her to implement the Gibson technique in her lab, but till now she has proven to be more of help than us! We'll prepare a new buffer ASAP. | ||
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| + | We did a PCR | ||
Day ends when Pollak throws coffee at Carla for some unknown reason. She believes he is conspiring against her (he hates her sweaters, breaks her watch and now the coffee episode). Pollak's defense: I just opened the door and there she was with the coffee. I didn't mean to do it. It just happened. | Day ends when Pollak throws coffee at Carla for some unknown reason. She believes he is conspiring against her (he hates her sweaters, breaks her watch and now the coffee episode). Pollak's defense: I just opened the door and there she was with the coffee. I didn't mean to do it. It just happened. | ||
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Friday: So... we had tech support from Olimex during the morning. Basically, they did the same as Juano, the light sensor terminals were soldered to small wires (in a more professional way of course). We like to call this sensor frankenstein's girlfriend :) (franky's chick). We'll try the arduino light sensing system next week. | Friday: So... we had tech support from Olimex during the morning. Basically, they did the same as Juano, the light sensor terminals were soldered to small wires (in a more professional way of course). We like to call this sensor frankenstein's girlfriend :) (franky's chick). We'll try the arduino light sensing system next week. | ||
| - | Attempted another Gibson to assemble C1.1, C1.2, Pcaa3 and ADF3 (check the Constructs section for more details on these parts). We occupied the isothermal buffer with the correct concentrations this time :) --> | + | Attempted another Gibson to assemble C1.1, C1.2, Pcaa3 and ADF3 (check the Constructs section for more details on these parts). We occupied the isothermal buffer with the correct concentrations this time :) Also, we purify bands from yesterday's PCR and check sizes of colony PCR done last yesterday. Some colonies are the right size. |
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Revision as of 19:52, 9 August 2012
