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Team:UC Chile/Cyano/Labook/week6
From 2012.igem.org
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(Created page with "{{Beerdragons}} <br> <p><font face="Calibri"><font size= "5">April 9 - April 15</font></font></p> <br> <p><font face="Calibri"><font size"4">") |
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<p><font face="Calibri"><font size= "5">April 9 - April 15</font></font></p> | <p><font face="Calibri"><font size= "5">April 9 - April 15</font></font></p> | ||
<br> | <br> | ||
| - | <p><font face="Calibri"><font size"4"> | + | <p><font face="Calibri"><font size"4">Monday</font></font></p> |
| + | <br> | ||
| + | <p><font face="Calibri"> | ||
| + | *PCR for C1 parts.</font></p> | ||
| + | <br> | ||
| + | <p><font face="Calibri"><font size="4">Tuesday</font></font></p> | ||
| + | <br> | ||
| + | <p><font face="Calibri"> | ||
| + | *PCR for C1. Problematic parts: KanR. | ||
| + | </font></p> | ||
| + | <br> | ||
| + | <p><font face="Calibri"><font size="4">Wednesday</font></font></p> | ||
| + | <br> | ||
| + | <p><font face="Calibri"> | ||
| + | *Started growth curve for our culture of Synechocystis PCC6803. | ||
| + | *Gel purification of previous PCR run. | ||
| + | </font></p> | ||
| + | <p><font face="Calibri"><font size="4">Friday</font></font></p> | ||
| + | <br> | ||
| + | <p><font face="Calibri"> | ||
| + | *PCR for C1 parts. | ||
| + | <br>Some conclusions: PCR and gel electrophoresis -> DNA from synechocystis used as template is not good enough. Genomic amplicons are not coming out right. As many PCR's have had bad results, we will use old PCR DNA as template and not genomic nor plasmid DNA.</font></p> | ||
Latest revision as of 20:43, 13 August 2012
April 9 - April 15
Monday
Tuesday
Wednesday
Friday
Some conclusions: PCR and gel electrophoresis -> DNA from synechocystis used as template is not good enough. Genomic amplicons are not coming out right. As many PCR's have had bad results, we will use old PCR DNA as template and not genomic nor plasmid DNA.
