Team:UC Chile/Cyano/Labook/week6

From 2012.igem.org

Cyanolux & Bactomithril - Pontificia Universidad Católica de Chile, iGEM 2012


April 9 - April 15


Monday


  • PCR for C1 parts.


Tuesday


  • PCR for C1. Problematic parts: KanR.


Wednesday


  • Started growth curve for our culture of Synechocystis PCC6803.
  • Gel purification of previous PCR run.

Friday


  • PCR for C1 parts.

Some conclusions: PCR and gel electrophoresis -> DNA from synechocystis used as template is not good enough. Genomic amplicons are not coming out right. As many PCR's have had bad results, we will use old PCR DNA as template and not genomic nor plasmid DNA.

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