Team:Tianjin/Notebook

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==内容部分的测试，多标题与目录浮动测试==
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2012年7月21日，第十一届中国国际合唱节圆满落下帷幕，天津大学北洋合唱团凭借原创作品《三十幅》和拉脱维亚作品《祈求》两首合唱曲目，在166支国内外优秀的合唱团中脱颖而出，一举夺得了成人混声组金奖，优秀钢琴伴奏奖第一名的好成绩，在北洋的众多荣誉中又增添了浓墨重彩的一笔。
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中国国际合唱节创办于1992年，今年时逢20周年，本届国际合唱节报名团体达166支，境外团体32支，其中有九支全球顶级的合唱团，评委、团员来自五大洲39个国家和地区；国内团体134支，来自23个省、市、自治区，总参赛人数近万人。这是自1992年该节创办以来规模最大、人数最多的一届，天津大学北洋合唱团受邀参加这次的比赛，也充分体现了合唱团在合唱界的水准和影响力。
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北洋合唱团十分重视这次比赛，无论是指挥还是团员们，在得知即将参加这次国际合唱节后，都放弃了假期，每天分为上午和下午两个单元进行集训，晚上也经常加班加点的排练，希望有一个饱满的精神状态，并在做好充分准备的情况下参加比赛，在每一个音符上每一个力度上都仔细揣摩，认真分析，力求将一中一洋两首作品完美演绎。
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在经过了半个多月紧张的排练后，北洋合唱团迎来了自己的比赛。2012年7月17日中午，合唱团抵达北京，在稍作休整后，团长便将大家集合起来进行比赛前的最后一次发声练习和曲目排练，北洋合唱团常任指挥任宝平老师也一直鼓励大家，并让大家放松心态，最后一次强调曲目中需要注意的地方。下午5点，合唱团到达中央民族音乐厅，在组委会的协助下进行走台和彩排。晚上7点，比赛正式开始，天津大学北洋合唱团作为当天成人混声D组的第一支队伍，用一首北洋原创的曲目《三十幅》拉开了比赛的序幕。
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《三十幅》由北洋合唱团驻团作曲家崔薇老师创作，歌词选自道家创始人老子的《道德经》，道家的“无”的思想和精神在这首作品中得到了很好的阐释，歌词与旋律相得益彰，因此在演唱时需要对歌词有深刻的理解，技巧上和音准上的要求也很高，只有这种高要求才能将这首作品中所蕴含的中国传统思想中内在的东西表现出来。北洋在这点上做的很到位，在台上将这首作品演绎的淋漓尽致，开场就博得了满堂喝彩。
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接下来是一首拉脱维亚作品《祈求》，这部作品是根据格列高素歌的元素所创作的现代的、高难度的合唱作品，有很大的演唱难度。整首作品分为三部分，每一部分的难度都很高,在排练的过程中遇到了很多的困难,包括和乐器的配合上,力度的协调上等等,因此在演唱时对作品的把握很困难,平时的排练也着重的强调了这些问题,每一位团员都很敬业,不断地揣摩,纠正发音,比赛当天,合唱团发挥出了正常的水平,平时一次次强调的问题在舞台上都没有出现,大家的努力最终没有白费.这首作品演绎的很完美.
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正是由于勤勤恳恳的排练和比赛时正常的发挥,天津大学北洋合唱团在166支国内外优秀的团队中夺得了成人混声组的金奖,并且获得了优秀钢琴伴奏奖的第一名，合唱团出色的表现赢得了国内外评委的高度赞扬，很多国际知名的评委在看到北洋的演出后都表示，“北洋音色”是走在世界的最前端的，北洋合唱团是这次比赛中他们所看到的最出色的合唱团。
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在即将到来的2012韩国丽水世博会中,北洋合唱团将代表中国赴韩国参加演出,期待他们更加精彩的表现..
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<p class="menu_head">Notebook Contents</p>
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<a href="https://2012.igem.org/Team:Tianjin/Notebook/Modeling">Modeling Notebook</a>
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<a href="https://2012.igem.org/Team:Tianjin/Notebook/HumanPractice">Human Practice Notebook</a>
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 +
        <div class="Calender-com">
 +
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 +
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 +
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 +
<div id="text-content">
 +
<center><span style="font-size:46px;font-family:Cambria;margin-top:10px;line-height:80%">Notes of Experiments</span></center>
 +
==July 6th, 2012==
 +
#We discuss our all of the possible projects, and finally choose one. And then allocate works to specific member.
 +
#Do some pre-experiment and make some reagants.
 +
##Liquid LB
 +
###10g tryptone
 +
###5g yeast extract
 +
###10g NaCl
 +
##Solid LB
 +
###10g tryptone
 +
###5g yeast extract
 +
###10g NaCl
 +
###20g agar
 +
#Cleaned up the lab and laboratory instruments
 +
 
 +
==July 8th, 2012==
 +
#Solutions for extracting plasmid.
 +
##50mM Glucose / 25mM Tris-Cl / 10mM EDTA，pH=8.0
 +
##0.2N NaOH / 1% SDS
 +
##3M KOAc / 2M HOAc
 +
#Experiment technologies training, such as making gel, gel electrophoresis.
 +
[[File:TJU2012-Note-gel-1.png|center|350px|gel 1]]
 +
 
 +
 
 +
[[File:TJU2012-Note-gel-2.png|center|350px|gel 2]]
 +
 
 +
==July 11th, 2012==
 +
#Designed primers and sent orders.
 +
#Optimized the project and allocated work.
 +
#Experiment technologies and knowledge training
 +
##PCR principle and operation
 +
##Gel Extraction
 +
[[File:TJU2012-Note-gel-3.png|center|350px|gel 3]]
 +
 
 +
 
 +
[[File:TJU2012-Note-gel-4.png|center|350px|gel 4]]
 +
 
 +
==July 13th, 2012==
 +
1. Received the oligo and began to mutate 16S rrnB operator.
 +
[[File:TJU2012-Note-gen-1.png|center|350px|Gene1]]
 +
2. Mutated RBS of RFP and checked through gel electrophoresis.
 +
[[File:TJU2012-Note-gen-2.png|center|250px|Gene2]]
 +
[[File:TJU2012-Note-gel-5.png|thumb|center|250px|~1800bp, constant with anticipation]]
 +
 
 +
==July 14th, 2012==
 +
#Transform two plasmids of we got yestday together into E.coli.[[File:TJU2012-Note-gen-3.png|center|500px|Gene 3]]
 +
#PCR verification of colonies on the LB plates.
 +
#Inculcated the right colonies in Liquid LB.
 +
 
 +
==July 15th, 2012==
 +
#Extract plasmid of cells inculcated after one day.
 +
#Enzyme test of the extracted plasmid.[[File:TJU2012-Note-gel-6.png|center|200px|Gel 6]]
 +
#Inclucated the right cells in Liquid LB and added Ala partly.
 +
 
 +
==July 20th, 2012==
 +
Analyse the results.
 +
[[File:TJU2012-Note-form-1.png|center|500px|Form 1]]
 +
[[File:TJU2012-Note-fig-2.png|center|300px|Fig 2]]
 +
 
 +
==July 24th, 2012==
 +
#Linked the GRP gene and O-RBS RFP gene. Gel extract the previous product again, and ligate using T4 with the following gradient.[[File:TJU2012-Note-form-2.png|center|500px|form 2]]
 +
#In the same method to construct three other systems.
 +
[[File:TJU2012-Mode-cal-fig-2.png|center|500px|fig 3]]
 +
[[File:TJU2012-Mode-cal-fig-3.png|center|500px|fig 4]]
 +
[[File:TJU2012-Mode-cal-fig-4.png|center|500px|fig 5]]
 +
 
 +
==July 30th, 2012==
 +
#Learned Fluorescence spectrophotometer.
 +
#measure the strength of GFP and RFP.
 +
[[File:TJU2012-Note-form-3.png|center|500px|form 3]]
 +
 
 +
==Aug. 2nd, 2012==
 +
#mutated the RBS of the Amp resistance gene.[[File:TJU2012-Note-gen-4.png|center|250px|Gene 4]]
 +
#linked genes and transformed them into E. coli.
 +
 
 +
==Aug. 4th, 2012==
 +
Results of plates
 +
[[File:TJU2012-Note-fig-3.png|center|300px|fig 3]]
 +
[[File:TJU2012-Note-form-4.png|center|500px|form 4]]
 +
 
 +
==Aug. 8th, 2012==
 +
Began to construct the five parts needed in Assembler in Yeast. Part 1 and part 2.
 +
 
 +
==Aug. 11th, 2012==
 +
Verified part 1 and 2. Synthesized the two small parts and linked through overlap PCR. Verified through ligase digestion and then gel electrophoresis.
 +
[[File:TJU2012-Note-fig-4.png|center|600px|fig 4]]
 +
 
 +
 
 +
[[File:TJU2012-Note-fig-5.png|center|500px|fig 5]]
 +
 
 +
==Aug. 12th, 2012==
 +
Began to build part 3, 4 and 5. Synthesized the two small parts and linked through overlap PCR.
 +
 
 +
==Aug. 14th, 2012==
 +
Verify part 3, 4 and 5. Verified through ligase digestion and then gel electrophoresis.
 +
[[File:TJU2012-Note-fig-6.png|center|500px|fig 6]]
 +
 
 +
 
 +
[[File:TJU2012-Note-fig-7.png|center|500px|fig 7]]
 +
 
 +
 
 +
[[File:TJU2012-Note-fig-8.png|center|500px|fig 8]]
 +
 
 +
==Aug. 18th, 2012==
 +
Transformed all the five parts into Yeast for Assembler in Yeast.
 +
 
 +
==Aug. 20th, 2012==
 +
#Results of the transformation.[[File:TJU2012-Note-fig-9.png|center|300px|fig 9]]
 +
#Extract plasmids from the Yeast.
 +
 
 +
==Aug. 22nd, 2012==
 +
#Transformed the plasmid from the Yeast into cells.[[File:TJU2012-Note-fig-10.png|thumb|center|300px|There are purple spots on the plates]]
 +
#Extracted plasmid and checked through gel electrophoresis.[[File:TJU2012-Note-gel-7.png|center|300px|gel 7]]
 +
#Some other results of Assembler in Yeast.
 +
[[File:TJU2012-Note-fig-11.png|center|300px|fig 11]]
 +
 
 +
 
 +
[[File:TJU2012-Note-fig-12.png|center|300px|fig 12]]
 +
 
 +
 
 +
[[File:TJU2012-Note-fig-13.png|center|300px|fig 13]]
 +
 
 +
 
 +
[[File:TJU2012-Note-fig-14.png|center|300px|fig 14]]
 +
 
 +
==Aug. 25th, 2012==
 +
#Began to build biobricks.[[File:Tianjin_BB2.PNG|center|500px|BB 2]]
 +
#Began to search articles on phages.
 +
 
 +
==Sept. 1st, 2012==
 +
Went to Peking University for exchanges.
 +
[[File:TJU2012-Note-fig-15.png|center|500px|fig 15]]
 +
 
 +
 
 +
[[File:TJU2012-Note-fig-16.png|center|500px|fig 16]]
 +
 
 +
==Sept. 2nd, 2012==
 +
#Found out phi X174 and firstly proposed our ideas.
 +
#The second sections of our biobricks.
 +
[[File:Tianjin_BB1.PNG|center|500px|BB 1]]
 +
 
 +
==Sept. 5th, 2012==
 +
#Some experiments on phages.
 +
#Optimized our biobricks.
 +
#Designed primers needed for mutating Phi X174 and send orders.
 +
 
 +
==Sept. 11th, 2012==
 +
#Began to mutate gene G and E.
 +
#Optimized our biobricks.
 +
 
 +
==Sept. 18th, 2012==
 +
Began to sort out our materials for wiki and upload the website.
 +
[[File:new1.jpg|center|500px|fig 17]]
 +
our team in Hongkong
 +
 
 +
 
 +
[[File:new2.jpg|center|500px|fig 18]]
 +
our gold award
 +
 
 +
 
 +
[[File:new3.jpg|center|500px|fig 19]]
 +
presentation in MIT
 +
 
 +
 
{{:Team:Tianjin/footer}}
{{:Team:Tianjin/footer}}

Latest revision as of 05:16, 9 May 2013

Notebook Contents

Modeling Notebook
Human Practice Notebook

July, 2012

Sun
Mon
Tue
Wed
Thu
Fri
Sat
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31

August, 2012

Sun
Mon
Tue
Wed
Thu
Fri
Sat
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31

September, 2012

Sun
Mon
Tue
Wed
Thu
Fri
Sat
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30

Notes of Experiments

July 6th, 2012

  1. We discuss our all of the possible projects, and finally choose one. And then allocate works to specific member.
  2. Do some pre-experiment and make some reagants.
    1. Liquid LB
      1. 10g tryptone
      2. 5g yeast extract
      3. 10g NaCl
    2. Solid LB
      1. 10g tryptone
      2. 5g yeast extract
      3. 10g NaCl
      4. 20g agar
  3. Cleaned up the lab and laboratory instruments

July 8th, 2012

  1. Solutions for extracting plasmid.
    1. 50mM Glucose / 25mM Tris-Cl / 10mM EDTA，pH=8.0
    2. 0.2N NaOH / 1% SDS
    3. 3M KOAc / 2M HOAc
  2. Experiment technologies training, such as making gel, gel electrophoresis.
gel 1


gel 2

July 11th, 2012

  1. Designed primers and sent orders.
  2. Optimized the project and allocated work.
  3. Experiment technologies and knowledge training
    1. PCR principle and operation
    2. Gel Extraction
gel 3


gel 4

July 13th, 2012

1. Received the oligo and began to mutate 16S rrnB operator.

Gene1

2. Mutated RBS of RFP and checked through gel electrophoresis.

Gene2
~1800bp, constant with anticipation

July 14th, 2012

  1. Transform two plasmids of we got yestday together into E.coli.
    Gene 3
  2. PCR verification of colonies on the LB plates.
  3. Inculcated the right colonies in Liquid LB.

July 15th, 2012

  1. Extract plasmid of cells inculcated after one day.
  2. Enzyme test of the extracted plasmid.
    Gel 6
  3. Inclucated the right cells in Liquid LB and added Ala partly.

July 20th, 2012

Analyse the results.

Form 1
Fig 2

July 24th, 2012

  1. Linked the GRP gene and O-RBS RFP gene. Gel extract the previous product again, and ligate using T4 with the following gradient.
    form 2
  2. In the same method to construct three other systems.
fig 3
fig 4
fig 5

July 30th, 2012

  1. Learned Fluorescence spectrophotometer.
  2. measure the strength of GFP and RFP.
form 3

Aug. 2nd, 2012

  1. mutated the RBS of the Amp resistance gene.
    Gene 4
  2. linked genes and transformed them into E. coli.

Aug. 4th, 2012

Results of plates

fig 3
form 4

Aug. 8th, 2012

Began to construct the five parts needed in Assembler in Yeast. Part 1 and part 2.

Aug. 11th, 2012

Verified part 1 and 2. Synthesized the two small parts and linked through overlap PCR. Verified through ligase digestion and then gel electrophoresis.

fig 4


fig 5

Aug. 12th, 2012

Began to build part 3, 4 and 5. Synthesized the two small parts and linked through overlap PCR.

Aug. 14th, 2012

Verify part 3, 4 and 5. Verified through ligase digestion and then gel electrophoresis.

fig 6


fig 7


fig 8

Aug. 18th, 2012

Transformed all the five parts into Yeast for Assembler in Yeast.

Aug. 20th, 2012

  1. Results of the transformation.
    fig 9
  2. Extract plasmids from the Yeast.

Aug. 22nd, 2012

  1. Transformed the plasmid from the Yeast into cells.
    There are purple spots on the plates
  2. Extracted plasmid and checked through gel electrophoresis.
    gel 7
  3. Some other results of Assembler in Yeast.
fig 11


fig 12


fig 13


fig 14

Aug. 25th, 2012

  1. Began to build biobricks.
    BB 2
  2. Began to search articles on phages.

Sept. 1st, 2012

Went to Peking University for exchanges.

fig 15


fig 16

Sept. 2nd, 2012

  1. Found out phi X174 and firstly proposed our ideas.
  2. The second sections of our biobricks.
BB 1

Sept. 5th, 2012

  1. Some experiments on phages.
  2. Optimized our biobricks.
  3. Designed primers needed for mutating Phi X174 and send orders.

Sept. 11th, 2012

  1. Began to mutate gene G and E.
  2. Optimized our biobricks.

Sept. 18th, 2012

Began to sort out our materials for wiki and upload the website.

fig 17

our team in Hongkong


fig 18

our gold award


fig 19

presentation in MIT


Retrieved from "http://2012.igem.org/Team:Tianjin/Notebook"