Team:Tianjin/Notebook

From 2012.igem.org

(Difference between revisions)
(July 11th, 2012)
(July 8th, 2012)
Line 24: Line 24:
##3M KOAc / 2M HOAc
##3M KOAc / 2M HOAc
#Experiment technologies training, such as making gel, gel electrophoresis.
#Experiment technologies training, such as making gel, gel electrophoresis.
-
[[File:TJU2012-Note-gel-1.png|center|gel 1]]
+
[[File:TJU2012-Note-gel-1.png|center|500px|gel 1]]
-
[[File:TJU2012-Note-gel-2.png|center|gel 2]]
+
[[File:TJU2012-Note-gel-2.png|center|500px|gel 2]]
==July 11th, 2012==
==July 11th, 2012==

Revision as of 18:42, 25 September 2012


Notes of Experiments

Contents

July 6th, 2012

  1. We discuss our all of the possible projects, and finally choose one. And then allocate works to specific member.
  2. Do some pre-experiment and make some reagants.
    1. Liquid LB
      1. 10g tryptone
      2. 5g yeast extract
      3. 10g NaCl
    2. Solid LB
      1. 10g tryptone
      2. 5g yeast extract
      3. 10g NaCl
      4. 20g agar
  3. Cleaned up the lab and laboratory instruments

July 8th, 2012

  1. Solutions for extracting plasmid.
    1. 50mM Glucose / 25mM Tris-Cl / 10mM EDTA,pH=8.0
    2. 0.2N NaOH / 1% SDS
    3. 3M KOAc / 2M HOAc
  2. Experiment technologies training, such as making gel, gel electrophoresis.
gel 1
gel 2

July 11th, 2012

  1. Designed primers and sent orders.
  2. Optimized the project and allocated work.
  3. Experiment technologies and knowledge training
    1. PCR principle and operation
    2. Gel Extraction
gel 3
gel 4

July 13th, 2012

1. Received the oligo and began to mutate 16S rrnB operator.

Gene1

2. Mutated RBS of RFP and checked through gel electrophoresis.

Gene2
~1800bp, constant with anticipation

July 14th, 2012

July 15th, 2012

July 20th, 2012

July 24th, 2012

July 30th, 2012

Aug. 2nd, 2012

Aug. 4th, 2012

Aug. 8th, 2012

Aug. 11th, 2012

Aug. 12th, 2012

Aug. 14th, 2012

Aug. 18nd, 2012

Aug. 20th, 2012

Aug. 22th, 2012

Aug. 25th, 2012

Sept. 1st, 2012

Sept. 2nd, 2012

Sept. 5st, 2012

Sept. 11st, 2012

Sept. 18st, 2012

</div>