Team:Technion/8 August 2012

From 2012.igem.org

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==Inbal==
==Inbal==
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Today I did PCR for the SP6 gene (2700bp, including primers) from the plasmid pACSP6R (using HF buffer). The product concentration was very low- 14/4 ng/ul, I ran the product in agarose gel and at 50bp was a small smear, apparently the primers.
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<br>
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In conclusion- the PCR didn't work. 
==Asaf==
==Asaf==
I mini-preped the starter I have made yesterday of Puc19 12.1 containing the RiboSwitch. <br>
I mini-preped the starter I have made yesterday of Puc19 12.1 containing the RiboSwitch. <br>
The DNA concentration is 154.5 ng/µl.
The DNA concentration is 154.5 ng/µl.
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==Hila==
==Hila==
==Lior==
==Lior==
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Cip- me and Noa minipreped the Cip gene for the sequencing. we got 189 ng/ul <br>
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Cip- me and Noa minipreped the Cip gene for the sequencing. we got 189 ng/ul. Moreover we made a starter for tomorrow. <br>
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xyIE- the transformation worked, but apparantly we left them for too long in the 37 degrees so we got colonies with satelites (the bacteria threw the plasmid away. so we took one colony that didnt had much satelites and made a starter for tomorrow.
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xyIE- the transformation worked, but apparantly we left them for too long in the 37 degrees so we got colonies with satelites (the bacteria threw the plasmid away). So we took one colony that didnt have much satelites and made a starter for tomorrow.
==Noa==
==Noa==
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==Rachel==
==Rachel==
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Today we ran PCR reaction, with suitable primers we had planned, for amplification of T7RNAP
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}}

Latest revision as of 13:11, 20 August 2012



Team:Technion/8_August_2012
March
MTWTFSS
      [http://2012.igem.org/wiki/index.php?title=Team:Technion/1_March_2012&preload=Team:Technion/Template:NewDate&action=edit 1] [http://2012.igem.org/wiki/index.php?title=Team:Technion/2_March_2012&preload=Team:Technion/Template:NewDate&action=edit 2] [http://2012.igem.org/wiki/index.php?title=Team:Technion/3_March_2012&preload=Team:Technion/Template:NewDate&action=edit 3] [http://2012.igem.org/wiki/index.php?title=Team:Technion/4_March_2012&preload=Team:Technion/Template:NewDate&action=edit 4]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/5_March_2012&preload=Team:Technion/Template:NewDate&action=edit 5] [http://2012.igem.org/wiki/index.php?title=Team:Technion/6_March_2012&preload=Team:Technion/Template:NewDate&action=edit 6] [http://2012.igem.org/wiki/index.php?title=Team:Technion/7_March_2012&preload=Team:Technion/Template:NewDate&action=edit 7] [http://2012.igem.org/wiki/index.php?title=Team:Technion/8_March_2012&preload=Team:Technion/Template:NewDate&action=edit 8] [http://2012.igem.org/wiki/index.php?title=Team:Technion/9_March_2012&preload=Team:Technion/Template:NewDate&action=edit 9] [http://2012.igem.org/wiki/index.php?title=Team:Technion/10_March_2012&preload=Team:Technion/Template:NewDate&action=edit 10] [http://2012.igem.org/wiki/index.php?title=Team:Technion/11_March_2012&preload=Team:Technion/Template:NewDate&action=edit 11]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/12_March_2012&preload=Team:Technion/Template:NewDate&action=edit 12] [http://2012.igem.org/wiki/index.php?title=Team:Technion/13_March_2012&preload=Team:Technion/Template:NewDate&action=edit 13] [http://2012.igem.org/wiki/index.php?title=Team:Technion/14_March_2012&preload=Team:Technion/Template:NewDate&action=edit 14] [http://2012.igem.org/wiki/index.php?title=Team:Technion/15_March_2012&preload=Team:Technion/Template:NewDate&action=edit 15] [http://2012.igem.org/wiki/index.php?title=Team:Technion/16_March_2012&preload=Team:Technion/Template:NewDate&action=edit 16] [http://2012.igem.org/wiki/index.php?title=Team:Technion/17_March_2012&preload=Team:Technion/Template:NewDate&action=edit 17] [http://2012.igem.org/wiki/index.php?title=Team:Technion/18_March_2012&preload=Team:Technion/Template:NewDate&action=edit 18]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/19_March_2012&preload=Team:Technion/Template:NewDate&action=edit 19] [http://2012.igem.org/wiki/index.php?title=Team:Technion/20_March_2012&preload=Team:Technion/Template:NewDate&action=edit 20] [http://2012.igem.org/Team:Technion/21_March_2012 21] [http://2012.igem.org/wiki/index.php?title=Team:Technion/22_March_2012&preload=Team:Technion/Template:NewDate&action=edit 22] [http://2012.igem.org/wiki/index.php?title=Team:Technion/23_March_2012&preload=Team:Technion/Template:NewDate&action=edit 23] [http://2012.igem.org/wiki/index.php?title=Team:Technion/24_March_2012&preload=Team:Technion/Template:NewDate&action=edit 24] [http://2012.igem.org/wiki/index.php?title=Team:Technion/25_March_2012&preload=Team:Technion/Template:NewDate&action=edit 25]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/26_March_2012&preload=Team:Technion/Template:NewDate&action=edit 26] [http://2012.igem.org/wiki/index.php?title=Team:Technion/27_March_2012&preload=Team:Technion/Template:NewDate&action=edit 27] [http://2012.igem.org/wiki/index.php?title=Team:Technion/28_March_2012&preload=Team:Technion/Template:NewDate&action=edit 28] [http://2012.igem.org/wiki/index.php?title=Team:Technion/29_March_2012&preload=Team:Technion/Template:NewDate&action=edit 29] [http://2012.igem.org/wiki/index.php?title=Team:Technion/30_March_2012&preload=Team:Technion/Template:NewDate&action=edit 30] [http://2012.igem.org/wiki/index.php?title=Team:Technion/31_March_2012&preload=Team:Technion/Template:NewDate&action=edit 31]
April
MTWTFSS
            [http://2012.igem.org/wiki/index.php?title=Team:Technion/1_April_2012&preload=Team:Technion/Template:NewDate&action=edit 1]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/2_April_2012&preload=Team:Technion/Template:NewDate&action=edit 2] [http://2012.igem.org/Team:Technion/3_April_2012 3] [http://2012.igem.org/Team:Technion/4_April_2012 4] [http://2012.igem.org/wiki/index.php?title=Team:Technion/5_April_2012&preload=Team:Technion/Template:NewDate&action=edit 5] [http://2012.igem.org/wiki/index.php?title=Team:Technion/6_April_2012&preload=Team:Technion/Template:NewDate&action=edit 6] [http://2012.igem.org/wiki/index.php?title=Team:Technion/7_April_2012&preload=Team:Technion/Template:NewDate&action=edit 7] [http://2012.igem.org/wiki/index.php?title=Team:Technion/8_April_2012&preload=Team:Technion/Template:NewDate&action=edit 8]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/9_April_2012&preload=Team:Technion/Template:NewDate&action=edit 9] [http://2012.igem.org/wiki/index.php?title=Team:Technion/10_April_2012&preload=Team:Technion/Template:NewDate&action=edit 10] [http://2012.igem.org/wiki/index.php?title=Team:Technion/11_April_2012&preload=Team:Technion/Template:NewDate&action=edit 11] [http://2012.igem.org/wiki/index.php?title=Team:Technion/12_April_2012&preload=Team:Technion/Template:NewDate&action=edit 12] [http://2012.igem.org/wiki/index.php?title=Team:Technion/13_April_2012&preload=Team:Technion/Template:NewDate&action=edit 13] [http://2012.igem.org/Team:Technion/14_April_2012 14] [http://2012.igem.org/wiki/index.php?title=Team:Technion/15_April_2012&preload=Team:Technion/Template:NewDate&action=edit 15]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/16_April_2012&preload=Team:Technion/Template:NewDate&action=edit 16] [http://2012.igem.org/wiki/index.php?title=Team:Technion/17_April_2012&preload=Team:Technion/Template:NewDate&action=edit 17] [http://2012.igem.org/wiki/index.php?title=Team:Technion/18_April_2012&preload=Team:Technion/Template:NewDate&action=edit 18] [http://2012.igem.org/wiki/index.php?title=Team:Technion/19_April_2012&preload=Team:Technion/Template:NewDate&action=edit 19] [http://2012.igem.org/wiki/index.php?title=Team:Technion/20_April_2012&preload=Team:Technion/Template:NewDate&action=edit 20] [http://2012.igem.org/wiki/index.php?title=Team:Technion/21_April_2012&preload=Team:Technion/Template:NewDate&action=edit 21] [http://2012.igem.org/wiki/index.php?title=Team:Technion/22_April_2012&preload=Team:Technion/Template:NewDate&action=edit 22]
[http://2012.igem.org/Team:Technion/23_April_2012 23] [http://2012.igem.org/wiki/index.php?title=Team:Technion/24_April_2012&preload=Team:Technion/Template:NewDate&action=edit 24] [http://2012.igem.org/Team:Technion/25_April_2012 25] [http://2012.igem.org/wiki/index.php?title=Team:Technion/26_April_2012&preload=Team:Technion/Template:NewDate&action=edit 26] [http://2012.igem.org/wiki/index.php?title=Team:Technion/27_April_2012&preload=Team:Technion/Template:NewDate&action=edit 27] [http://2012.igem.org/Team:Technion/28_April_2012 28] [http://2012.igem.org/wiki/index.php?title=Team:Technion/29_April_2012&preload=Team:Technion/Template:NewDate&action=edit 29]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/30_April_2012&preload=Team:Technion/Template:NewDate&action=edit 30]
May
MTWTFSS
  [http://2012.igem.org/Team:Technion/1_May_2012 1] [http://2012.igem.org/wiki/index.php?title=Team:Technion/2_May_2012&preload=Team:Technion/Template:NewDate&action=edit 2] [http://2012.igem.org/wiki/index.php?title=Team:Technion/3_May_2012&preload=Team:Technion/Template:NewDate&action=edit 3] [http://2012.igem.org/wiki/index.php?title=Team:Technion/4_May_2012&preload=Team:Technion/Template:NewDate&action=edit 4] [http://2012.igem.org/wiki/index.php?title=Team:Technion/5_May_2012&preload=Team:Technion/Template:NewDate&action=edit 5] [http://2012.igem.org/wiki/index.php?title=Team:Technion/6_May_2012&preload=Team:Technion/Template:NewDate&action=edit 6]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/7_May_2012&preload=Team:Technion/Template:NewDate&action=edit 7] [http://2012.igem.org/wiki/index.php?title=Team:Technion/8_May_2012&preload=Team:Technion/Template:NewDate&action=edit 8] [http://2012.igem.org/wiki/index.php?title=Team:Technion/9_May_2012&preload=Team:Technion/Template:NewDate&action=edit 9] [http://2012.igem.org/Team:Technion/10_May_2012 10] [http://2012.igem.org/wiki/index.php?title=Team:Technion/11_May_2012&preload=Team:Technion/Template:NewDate&action=edit 11] [http://2012.igem.org/wiki/index.php?title=Team:Technion/12_May_2012&preload=Team:Technion/Template:NewDate&action=edit 12] [http://2012.igem.org/wiki/index.php?title=Team:Technion/13_May_2012&preload=Team:Technion/Template:NewDate&action=edit 13]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/14_May_2012&preload=Team:Technion/Template:NewDate&action=edit 14] [http://2012.igem.org/wiki/index.php?title=Team:Technion/15_May_2012&preload=Team:Technion/Template:NewDate&action=edit 15] [http://2012.igem.org/wiki/index.php?title=Team:Technion/16_May_2012&preload=Team:Technion/Template:NewDate&action=edit 16] [http://2012.igem.org/wiki/index.php?title=Team:Technion/17_May_2012&preload=Team:Technion/Template:NewDate&action=edit 17] [http://2012.igem.org/wiki/index.php?title=Team:Technion/18_May_2012&preload=Team:Technion/Template:NewDate&action=edit 18] [http://2012.igem.org/wiki/index.php?title=Team:Technion/19_May_2012&preload=Team:Technion/Template:NewDate&action=edit 19] [http://2012.igem.org/wiki/index.php?title=Team:Technion/20_May_2012&preload=Team:Technion/Template:NewDate&action=edit 20]
[http://2012.igem.org/Team:Technion/21_May_2012 21] [http://2012.igem.org/wiki/index.php?title=Team:Technion/22_May_2012&preload=Team:Technion/Template:NewDate&action=edit 22] [http://2012.igem.org/wiki/index.php?title=Team:Technion/23_May_2012&preload=Team:Technion/Template:NewDate&action=edit 23] [http://2012.igem.org/wiki/index.php?title=Team:Technion/24_May_2012&preload=Team:Technion/Template:NewDate&action=edit 24] [http://2012.igem.org/wiki/index.php?title=Team:Technion/25_May_2012&preload=Team:Technion/Template:NewDate&action=edit 25] [http://2012.igem.org/wiki/index.php?title=Team:Technion/26_May_2012&preload=Team:Technion/Template:NewDate&action=edit 26] [http://2012.igem.org/wiki/index.php?title=Team:Technion/27_May_2012&preload=Team:Technion/Template:NewDate&action=edit 27]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/28_May_2012&preload=Team:Technion/Template:NewDate&action=edit 28] [http://2012.igem.org/wiki/index.php?title=Team:Technion/29_May_2012&preload=Team:Technion/Template:NewDate&action=edit 29] [http://2012.igem.org/wiki/index.php?title=Team:Technion/30_May_2012&preload=Team:Technion/Template:NewDate&action=edit 30] [http://2012.igem.org/wiki/index.php?title=Team:Technion/31_May_2012&preload=Team:Technion/Template:NewDate&action=edit 31]
June
MTWTFSS
        [http://2012.igem.org/wiki/index.php?title=Team:Technion/1_June_2012&preload=Team:Technion/Template:NewDate&action=edit 1] [http://2012.igem.org/wiki/index.php?title=Team:Technion/2_June_2012&preload=Team:Technion/Template:NewDate&action=edit 2] [http://2012.igem.org/Team:Technion/3_June_2012 3]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/4_June_2012&preload=Team:Technion/Template:NewDate&action=edit 4] [http://2012.igem.org/Team:Technion/5_June_2012 5] [http://2012.igem.org/Team:Technion/6_June_2012 6] [http://2012.igem.org/Team:Technion/7_June_2012 7] [http://2012.igem.org/wiki/index.php?title=Team:Technion/8_June_2012&preload=Team:Technion/Template:NewDate&action=edit 8] [http://2012.igem.org/wiki/index.php?title=Team:Technion/9_June_2012&preload=Team:Technion/Template:NewDate&action=edit 9] [http://2012.igem.org/Team:Technion/10_June_2012 10]
[http://2012.igem.org/Team:Technion/11_June_2012 11] [http://2012.igem.org/Team:Technion/12_June_2012 12] [http://2012.igem.org/Team:Technion/13_June_2012 13] [http://2012.igem.org/Team:Technion/14_June_2012 14] [http://2012.igem.org/wiki/index.php?title=Team:Technion/15_June_2012&preload=Team:Technion/Template:NewDate&action=edit 15] [http://2012.igem.org/wiki/index.php?title=Team:Technion/16_June_2012&preload=Team:Technion/Template:NewDate&action=edit 16] [http://2012.igem.org/Team:Technion/17_June_2012 17]
[http://2012.igem.org/Team:Technion/18_June_2012 18] [http://2012.igem.org/Team:Technion/19_June_2012 19] [http://2012.igem.org/Team:Technion/20_June_2012 20] [http://2012.igem.org/Team:Technion/21_June_2012 21] [http://2012.igem.org/wiki/index.php?title=Team:Technion/22_June_2012&preload=Team:Technion/Template:NewDate&action=edit 22] [http://2012.igem.org/wiki/index.php?title=Team:Technion/23_June_2012&preload=Team:Technion/Template:NewDate&action=edit 23] [http://2012.igem.org/Team:Technion/24_June_2012 24]
[http://2012.igem.org/Team:Technion/25_June_2012 25] [http://2012.igem.org/Team:Technion/26_June_2012 26] [http://2012.igem.org/Team:Technion/27_June_2012 27] [http://2012.igem.org/wiki/index.php?title=Team:Technion/28_June_2012&preload=Team:Technion/Template:NewDate&action=edit 28] [http://2012.igem.org/wiki/index.php?title=Team:Technion/29_June_2012&preload=Team:Technion/Template:NewDate&action=edit 29] [http://2012.igem.org/wiki/index.php?title=Team:Technion/30_June_2012&preload=Team:Technion/Template:NewDate&action=edit 30]
July
MTWTFSS
            [http://2012.igem.org/wiki/index.php?title=Team:Technion/1_July_2012&preload=Team:Technion/Template:NewDate&action=edit 1]
[http://2012.igem.org/Team:Technion/2_July_2012 2] [http://2012.igem.org/Team:Technion/3_July_2012 3] [http://2012.igem.org/Team:Technion/4_July_2012 4] [http://2012.igem.org/wiki/index.php?title=Team:Technion/5_July_2012&preload=Team:Technion/Template:NewDate&action=edit 5] [http://2012.igem.org/wiki/index.php?title=Team:Technion/6_July_2012&preload=Team:Technion/Template:NewDate&action=edit 6] [http://2012.igem.org/wiki/index.php?title=Team:Technion/7_July_2012&preload=Team:Technion/Template:NewDate&action=edit 7] [http://2012.igem.org/Team:Technion/8_July_2012 8]
[http://2012.igem.org/Team:Technion/9_July_2012 9] [http://2012.igem.org/Team:Technion/10_July_2012 10] [http://2012.igem.org/Team:Technion/11_July_2012 11] [http://2012.igem.org/wiki/index.php?title=Team:Technion/12_July_2012&preload=Team:Technion/Template:NewDate&action=edit 12] [http://2012.igem.org/wiki/index.php?title=Team:Technion/13_July_2012&preload=Team:Technion/Template:NewDate&action=edit 13] [http://2012.igem.org/wiki/index.php?title=Team:Technion/14_July_2012&preload=Team:Technion/Template:NewDate&action=edit 14] [http://2012.igem.org/wiki/index.php?title=Team:Technion/15_July_2012&preload=Team:Technion/Template:NewDate&action=edit 15]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/16_July_2012&preload=Team:Technion/Template:NewDate&action=edit 16] [http://2012.igem.org/Team:Technion/17_July_2012 17] [http://2012.igem.org/Team:Technion/18_July_2012 18] [http://2012.igem.org/Team:Technion/19_July_2012 19] [http://2012.igem.org/wiki/index.php?title=Team:Technion/20_July_2012&preload=Team:Technion/Template:NewDate&action=edit 20] [http://2012.igem.org/wiki/index.php?title=Team:Technion/21_July_2012&preload=Team:Technion/Template:NewDate&action=edit 21] [http://2012.igem.org/Team:Technion/22_July_2012 22]
[http://2012.igem.org/Team:Technion/23_July_2012 23] [http://2012.igem.org/Team:Technion/24_July_2012 24] [http://2012.igem.org/Team:Technion/25_July_2012 25] [http://2012.igem.org/Team:Technion/26_July_2012 26] [http://2012.igem.org/wiki/index.php?title=Team:Technion/27_July_2012&preload=Team:Technion/Template:NewDate&action=edit 27] [http://2012.igem.org/wiki/index.php?title=Team:Technion/28_July_2012&preload=Team:Technion/Template:NewDate&action=edit 28] [http://2012.igem.org/Team:Technion/29_July_2012 29]
[http://2012.igem.org/Team:Technion/30_July_2012 30] [http://2012.igem.org/Team:Technion/31_July_2012 31]
August
MTWTFSS
    [http://2012.igem.org/Team:Technion/1_August_2012 1] [http://2012.igem.org/Team:Technion/2_August_2012 2] [http://2012.igem.org/wiki/index.php?title=Team:Technion/3_August_2012&preload=Team:Technion/Template:NewDate&action=edit 3] [http://2012.igem.org/Team:Technion/4_August_2012 4] [http://2012.igem.org/Team:Technion/5_August_2012 5]
[http://2012.igem.org/Team:Technion/6_August_2012 6] [http://2012.igem.org/Team:Technion/7_August_2012 7] [http://2012.igem.org/Team:Technion/8_August_2012 8] [http://2012.igem.org/Team:Technion/9_August_2012 9] [http://2012.igem.org/wiki/index.php?title=Team:Technion/10_August_2012&preload=Team:Technion/Template:NewDate&action=edit 10] [http://2012.igem.org/wiki/index.php?title=Team:Technion/11_August_2012&preload=Team:Technion/Template:NewDate&action=edit 11] [http://2012.igem.org/Team:Technion/12_August_2012 12]
[http://2012.igem.org/Team:Technion/13_August_2012 13] [http://2012.igem.org/Team:Technion/14_August_2012 14] [http://2012.igem.org/Team:Technion/15_August_2012 15] [http://2012.igem.org/Team:Technion/16_August_2012 16] [http://2012.igem.org/Team:Technion/17_August_2012 17] [http://2012.igem.org/Team:Technion/18_August_2012 18] [http://2012.igem.org/Team:Technion/19_August_2012 19]
[http://2012.igem.org/Team:Technion/20_August_2012 20] [http://2012.igem.org/Team:Technion/21_August_2012 21] [http://2012.igem.org/Team:Technion/22_August_2012 22] [http://2012.igem.org/Team:Technion/23_August_2012 23] [http://2012.igem.org/Team:Technion/24_August_2012 24] [http://2012.igem.org/wiki/index.php?title=Team:Technion/25_August_2012&preload=Team:Technion/Template:NewDate&action=edit 25] [http://2012.igem.org/Team:Technion/26_August_2012 26]
[http://2012.igem.org/Team:Technion/27_August_2012 27] [http://2012.igem.org/Team:Technion/28_August_2012 28] [http://2012.igem.org/Team:Technion/29_August_2012 29] [http://2012.igem.org/Team:Technion/30_August_2012 30] [http://2012.igem.org/Team:Technion/31_August_2012 31]
September
MTWTFSS
          [http://2012.igem.org/Team:Technion/1_September_2012 1] [http://2012.igem.org/Team:Technion/2_September_2012 2]
[http://2012.igem.org/Team:Technion/3_September_2012 3] [http://2012.igem.org/Team:Technion/4_September_2012 4] [http://2012.igem.org/Team:Technion/5_September_2012 5] [http://2012.igem.org/Team:Technion/6_September_2012 6] [http://2012.igem.org/Team:Technion/7_September_2012 7] [http://2012.igem.org/Team:Technion/8_September_2012 8] [http://2012.igem.org/Team:Technion/9_September_2012 9]
[http://2012.igem.org/Team:Technion/10_September_2012 10] [http://2012.igem.org/Team:Technion/11_September_2012 11] [http://2012.igem.org/Team:Technion/12_September_2012 12] [http://2012.igem.org/Team:Technion/13_September_2012 13] [http://2012.igem.org/Team:Technion/14_September_2012 14] [http://2012.igem.org/Team:Technion/15_September_2012 15] [http://2012.igem.org/Team:Technion/16_September_2012 16]
[http://2012.igem.org/Team:Technion/17_September_2012 17] [http://2012.igem.org/Team:Technion/18_September_2012 18] [http://2012.igem.org/Team:Technion/19_September_2012 19] [http://2012.igem.org/Team:Technion/20_September_2012 20] [http://2012.igem.org/Team:Technion/21_September_2012 21] [http://2012.igem.org/Team:Technion/22_September_2012 22] [http://2012.igem.org/Team:Technion/23_September_2012 23]
[http://2012.igem.org/Team:Technion/24_September_2012 24] [http://2012.igem.org/Team:Technion/25_September_2012 25] [http://2012.igem.org/Team:Technion/26_September_2012 26] [http://2012.igem.org/wiki/index.php?title=Team:Technion/27_September_2012&preload=Team:Technion/Template:NewDate&action=edit 27] [http://2012.igem.org/wiki/index.php?title=Team:Technion/28_September_2012&preload=Team:Technion/Template:NewDate&action=edit 28] [http://2012.igem.org/wiki/index.php?title=Team:Technion/29_September_2012&preload=Team:Technion/Template:NewDate&action=edit 29] [http://2012.igem.org/wiki/index.php?title=Team:Technion/30_September_2012&preload=Team:Technion/Template:NewDate&action=edit 30]
October
MTWTFSS
[http://2012.igem.org/wiki/index.php?title=Team:Technion/1_October_2012&preload=Team:Technion/Template:NewDate&action=edit 1] [http://2012.igem.org/wiki/index.php?title=Team:Technion/2_October_2012&preload=Team:Technion/Template:NewDate&action=edit 2] [http://2012.igem.org/wiki/index.php?title=Team:Technion/3_October_2012&preload=Team:Technion/Template:NewDate&action=edit 3] [http://2012.igem.org/wiki/index.php?title=Team:Technion/4_October_2012&preload=Team:Technion/Template:NewDate&action=edit 4] [http://2012.igem.org/wiki/index.php?title=Team:Technion/5_October_2012&preload=Team:Technion/Template:NewDate&action=edit 5] [http://2012.igem.org/wiki/index.php?title=Team:Technion/6_October_2012&preload=Team:Technion/Template:NewDate&action=edit 6] [http://2012.igem.org/wiki/index.php?title=Team:Technion/7_October_2012&preload=Team:Technion/Template:NewDate&action=edit 7]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/8_October_2012&preload=Team:Technion/Template:NewDate&action=edit 8] [http://2012.igem.org/wiki/index.php?title=Team:Technion/9_October_2012&preload=Team:Technion/Template:NewDate&action=edit 9] [http://2012.igem.org/wiki/index.php?title=Team:Technion/10_October_2012&preload=Team:Technion/Template:NewDate&action=edit 10] [http://2012.igem.org/wiki/index.php?title=Team:Technion/11_October_2012&preload=Team:Technion/Template:NewDate&action=edit 11] [http://2012.igem.org/wiki/index.php?title=Team:Technion/12_October_2012&preload=Team:Technion/Template:NewDate&action=edit 12] [http://2012.igem.org/wiki/index.php?title=Team:Technion/13_October_2012&preload=Team:Technion/Template:NewDate&action=edit 13] [http://2012.igem.org/wiki/index.php?title=Team:Technion/14_October_2012&preload=Team:Technion/Template:NewDate&action=edit 14]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/15_October_2012&preload=Team:Technion/Template:NewDate&action=edit 15] [http://2012.igem.org/wiki/index.php?title=Team:Technion/16_October_2012&preload=Team:Technion/Template:NewDate&action=edit 16] [http://2012.igem.org/wiki/index.php?title=Team:Technion/17_October_2012&preload=Team:Technion/Template:NewDate&action=edit 17] [http://2012.igem.org/wiki/index.php?title=Team:Technion/18_October_2012&preload=Team:Technion/Template:NewDate&action=edit 18] [http://2012.igem.org/wiki/index.php?title=Team:Technion/19_October_2012&preload=Team:Technion/Template:NewDate&action=edit 19] [http://2012.igem.org/wiki/index.php?title=Team:Technion/20_October_2012&preload=Team:Technion/Template:NewDate&action=edit 20] [http://2012.igem.org/wiki/index.php?title=Team:Technion/21_October_2012&preload=Team:Technion/Template:NewDate&action=edit 21]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/22_October_2012&preload=Team:Technion/Template:NewDate&action=edit 22] [http://2012.igem.org/wiki/index.php?title=Team:Technion/23_October_2012&preload=Team:Technion/Template:NewDate&action=edit 23] [http://2012.igem.org/wiki/index.php?title=Team:Technion/24_October_2012&preload=Team:Technion/Template:NewDate&action=edit 24] [http://2012.igem.org/wiki/index.php?title=Team:Technion/25_October_2012&preload=Team:Technion/Template:NewDate&action=edit 25] [http://2012.igem.org/wiki/index.php?title=Team:Technion/26_October_2012&preload=Team:Technion/Template:NewDate&action=edit 26] [http://2012.igem.org/wiki/index.php?title=Team:Technion/27_October_2012&preload=Team:Technion/Template:NewDate&action=edit 27] [http://2012.igem.org/wiki/index.php?title=Team:Technion/28_October_2012&preload=Team:Technion/Template:NewDate&action=edit 28]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/29_October_2012&preload=Team:Technion/Template:NewDate&action=edit 29] [http://2012.igem.org/wiki/index.php?title=Team:Technion/30_October_2012&preload=Team:Technion/Template:NewDate&action=edit 30] [http://2012.igem.org/wiki/index.php?title=Team:Technion/31_October_2012&preload=Team:Technion/Template:NewDate&action=edit 31]
November
MTWTFSS
      [http://2012.igem.org/wiki/index.php?title=Team:Technion/1_November_2012&preload=Team:Technion/Template:NewDate&action=edit 1] [http://2012.igem.org/wiki/index.php?title=Team:Technion/2_November_2012&preload=Team:Technion/Template:NewDate&action=edit 2] [http://2012.igem.org/wiki/index.php?title=Team:Technion/3_November_2012&preload=Team:Technion/Template:NewDate&action=edit 3] [http://2012.igem.org/wiki/index.php?title=Team:Technion/4_November_2012&preload=Team:Technion/Template:NewDate&action=edit 4]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/5_November_2012&preload=Team:Technion/Template:NewDate&action=edit 5] [http://2012.igem.org/wiki/index.php?title=Team:Technion/6_November_2012&preload=Team:Technion/Template:NewDate&action=edit 6] [http://2012.igem.org/wiki/index.php?title=Team:Technion/7_November_2012&preload=Team:Technion/Template:NewDate&action=edit 7] [http://2012.igem.org/wiki/index.php?title=Team:Technion/8_November_2012&preload=Team:Technion/Template:NewDate&action=edit 8] [http://2012.igem.org/wiki/index.php?title=Team:Technion/9_November_2012&preload=Team:Technion/Template:NewDate&action=edit 9] [http://2012.igem.org/wiki/index.php?title=Team:Technion/10_November_2012&preload=Team:Technion/Template:NewDate&action=edit 10] [http://2012.igem.org/wiki/index.php?title=Team:Technion/11_November_2012&preload=Team:Technion/Template:NewDate&action=edit 11]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/12_November_2012&preload=Team:Technion/Template:NewDate&action=edit 12] [http://2012.igem.org/wiki/index.php?title=Team:Technion/13_November_2012&preload=Team:Technion/Template:NewDate&action=edit 13] [http://2012.igem.org/wiki/index.php?title=Team:Technion/14_November_2012&preload=Team:Technion/Template:NewDate&action=edit 14] [http://2012.igem.org/wiki/index.php?title=Team:Technion/15_November_2012&preload=Team:Technion/Template:NewDate&action=edit 15] [http://2012.igem.org/wiki/index.php?title=Team:Technion/16_November_2012&preload=Team:Technion/Template:NewDate&action=edit 16] [http://2012.igem.org/wiki/index.php?title=Team:Technion/17_November_2012&preload=Team:Technion/Template:NewDate&action=edit 17] [http://2012.igem.org/wiki/index.php?title=Team:Technion/18_November_2012&preload=Team:Technion/Template:NewDate&action=edit 18]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/19_November_2012&preload=Team:Technion/Template:NewDate&action=edit 19] [http://2012.igem.org/wiki/index.php?title=Team:Technion/20_November_2012&preload=Team:Technion/Template:NewDate&action=edit 20] [http://2012.igem.org/wiki/index.php?title=Team:Technion/21_November_2012&preload=Team:Technion/Template:NewDate&action=edit 21] [http://2012.igem.org/wiki/index.php?title=Team:Technion/22_November_2012&preload=Team:Technion/Template:NewDate&action=edit 22] [http://2012.igem.org/wiki/index.php?title=Team:Technion/23_November_2012&preload=Team:Technion/Template:NewDate&action=edit 23] [http://2012.igem.org/wiki/index.php?title=Team:Technion/24_November_2012&preload=Team:Technion/Template:NewDate&action=edit 24] [http://2012.igem.org/wiki/index.php?title=Team:Technion/25_November_2012&preload=Team:Technion/Template:NewDate&action=edit 25]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/26_November_2012&preload=Team:Technion/Template:NewDate&action=edit 26] [http://2012.igem.org/wiki/index.php?title=Team:Technion/27_November_2012&preload=Team:Technion/Template:NewDate&action=edit 27] [http://2012.igem.org/wiki/index.php?title=Team:Technion/28_November_2012&preload=Team:Technion/Template:NewDate&action=edit 28] [http://2012.igem.org/wiki/index.php?title=Team:Technion/29_November_2012&preload=Team:Technion/Template:NewDate&action=edit 29] [http://2012.igem.org/wiki/index.php?title=Team:Technion/30_November_2012&preload=Team:Technion/Template:NewDate&action=edit 30]


Ilya

- Checked the sequencing results for BBa I0462. There is no way it is BBa I0462. A couple of days ago, I noticed that the experience info about this BioBrick states that it is actually a different BioBrick. Trying to clone this part was a waste of time. I will amplify the correct BBa I0462 from BBa F2620 using PCR and clone it downstream to R0062+RS+mCh.
- Glycerol stock of BBa F2620. Freezer position is 3C.
- Miniprep of BBa F2620. Concentrations are: 1F - 168.5 ng/ul, 2F - 169.5 ng/ul.
- The plate reader results seem to be weird. There is no change with concentration and the auto-fluorescence seems to be very high. Maybe the riboswitch doesn't function as expected.

Inbal

Today I did PCR for the SP6 gene (2700bp, including primers) from the plasmid pACSP6R (using HF buffer). The product concentration was very low- 14/4 ng/ul, I ran the product in agarose gel and at 50bp was a small smear, apparently the primers.
In conclusion- the PCR didn't work.

Asaf

I mini-preped the starter I have made yesterday of Puc19 12.1 containing the RiboSwitch.
The DNA concentration is 154.5 ng/µl.

Hila

Lior

Cip- me and Noa minipreped the Cip gene for the sequencing. we got 189 ng/ul. Moreover we made a starter for tomorrow.
xyIE- the transformation worked, but apparantly we left them for too long in the 37 degrees so we got colonies with satelites (the bacteria threw the plasmid away). So we took one colony that didnt have much satelites and made a starter for tomorrow.

Noa

Evgeni

Shahar

Rachel

Today we ran PCR reaction, with suitable primers we had planned, for amplification of T7RNAP