Team:Technion/8 August 2012
From 2012.igem.org
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==Asaf== | ==Asaf== | ||
- | I mini- | + | I mini-preped the starter I have made yesterday of Puc19 12.1 containing the RiboSwitch. <br> |
The DNA concentration is 154.5 ng/µl. | The DNA concentration is 154.5 ng/µl. | ||
Revision as of 07:13, 9 August 2012
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Ilya
- Checked the sequencing results for BBa I0462. There is no way it is BBa I0462. A couple of days ago, I noticed that the experience info about this BioBrick states that it is actually a different BioBrick. Trying to clone this part was a waste of time. I will amplify the correct BBa I0462 from BBa F2620 using PCR and clone it downstream to R0062+RS+mCh.
- Glycerol stock of BBa F2620. Freezer position is 3C.
- Miniprep of BBa F2620. Concentrations are: 1F - 168.5 ng/ul, 2F - 169.5 ng/ul.
- The plate reader results seem to be weird. There is no change with concentration and the auto-fluorescence seems to be very high. Maybe the riboswitch doesn't function as expected.
Inbal
Asaf
I mini-preped the starter I have made yesterday of Puc19 12.1 containing the RiboSwitch.
The DNA concentration is 154.5 ng/µl.